来自深层地下细菌鞘氨醇单胞菌属菌株F199的一种分解代谢质粒的物理图谱绘制与特性分析。
Physical mapping and characterization of a catabolic plasmid from the deep-subsurface bacterium Sphingomonas sp. strain F199.
作者信息
Stillwell L C, Thurston S J, Schneider R P, Romine M F, Fredrickson J K, Saffer J D
机构信息
Pacific Northwest Laboratory, Richland, Washington 99352, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4537-9. doi: 10.1128/jb.177.15.4537-4539.1995.
Abstract
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid library indicated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in this Sphingomonas strain are, at least in part, plasmid encoded.
摘要
一种超螺旋的180 kb质粒pNL1已从深层地下的化学异养鞘氨醇单胞菌属菌株F199中分离出来，并构建了其物理图谱。对源自pNL1的黏粒文库的分析表明，儿茶酚2,3-双加氧酶活性与质粒的两个不同区域相关联。因此，该鞘氨醇单胞菌菌株中芳香族化合物分解代谢的基因至少部分是由质粒编码的。
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