抗组蛋白抗体的酶联免疫吸附测定及其在系统性红斑狼疮血清中的存在情况。
Enzyme-linked immunosorbent assay for anti-histone antibodies and their presence in systemic lupus erythematosus sera.
作者信息
Aitkaci A, Monier J C, Mamelle N
出版信息
J Immunol Methods. 1981;44(3):311-22. doi: 10.1016/0022-1759(81)90049-1.
We describe an enzyme-linked immunosorbent assay (ELISA) with adsorption of histones (total and fractions) on glass beads and saturation of excess sites with sheep serum. The anti-histone antibodies are detected with peroxidase conjugate and developed with Trinder's reagent which has great stability. This very sensitive method detects anti-histone antibodies in 53% of SLE patients and in virtually no other diseases. Positive reactions are observed only with total histones and fractions H1 and H2b.
我们描述了一种酶联免疫吸附测定法（ELISA），该方法将组蛋白（总组蛋白和各组分）吸附在玻璃珠上，并用羊血清使多余位点饱和。用辣根过氧化物酶结合物检测抗组蛋白抗体，并用稳定性极佳的Trinder试剂显色。这种非常灵敏的方法在53%的系统性红斑狼疮（SLE）患者中检测到抗组蛋白抗体，而在几乎其他疾病中未检测到。仅在总组蛋白以及H1和H2b组分中观察到阳性反应。
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