Irving C S, Lapidot A
Antimicrob Agents Chemother. 1978 Nov;14(5):695-703. doi: 10.1128/AAC.14.5.695.
The effects of binding and the bactericidal action of vancomycin on the arrangement and mobilities of cell wall polymers in Bacillus licheniformis were investigated by (15)N nuclear magnetic resonance spectroscopy. The bactericidal action of vancomycin led to reduced mobilities of cell wall teichoic acid and teichuronic acid in surviving cells. The decrease in teichoic acid mobility was also observed upon binding of vancomycin to B. licheniformis cells and resulted from a specific interaction between the antibiotic and teichoic acid, rather than from electrostatic contraction of the cell wall. The reduction in teichuronic acid mobility appeared to be related either to the elastic contraction of the cell wall resulting from loss of cell turgor or to separation of the cell wall from the protoplast membrane. No spectral changes associated with cell wall autolysis or alterations in cell wall composition, amidation, and cross-linking were found in vancomycin-treated B. licheniformis cells. Binding of vancomycin to Micrococcus lysodeikticus cell walls led to a decrease in mobility of C-terminal d-alanine residues but was accompanied by an increase in the mobilities of other peptidoglycan residues. The possible contributions of changes in the arrangements of cell wall polymers to the lethal action of vancomycin is discussed.
通过¹⁵N核磁共振光谱研究了万古霉素的结合作用及其对 licheniformis芽孢杆菌细胞壁聚合物排列和流动性的杀菌作用。万古霉素的杀菌作用导致存活细胞中细胞壁磷壁酸和磷壁醛酸的流动性降低。当万古霉素与licheniformis芽孢杆菌细胞结合时，也观察到磷壁酸流动性的降低，这是由于抗生素与磷壁酸之间的特异性相互作用，而不是由于细胞壁的静电收缩。磷壁醛酸流动性的降低似乎与细胞膨压丧失导致的细胞壁弹性收缩或细胞壁与原生质膜分离有关。在万古霉素处理的licheniformis芽孢杆菌细胞中，未发现与细胞壁自溶或细胞壁组成、酰胺化和交联改变相关的光谱变化。万古霉素与溶壁微球菌细胞壁的结合导致C末端d-丙氨酸残基的流动性降低，但同时其他肽聚糖残基的流动性增加。讨论了细胞壁聚合物排列变化对万古霉素致死作用的可能贡献。
