Bulgin M S, Ward A C, Barrett D P, Lane V M
Can Vet J. 1989 Mar;30(3):235-9.
Fecal samples were taken at the time of pregnancy examinations and at parturition from two beef herds. They were also taken from sick calves at the onset of disease, and from 25% of the healthy calves at 15 days of age. All fecal samples were examined by electron microscopy for viruses.Four cows in herd A were detected excreting coronavirus, one at the time of the pregnancy examinations and three at parturition. The first cow was removed from the herd and the others calved at the end of the season. There were no sick calves.No cows in herd B were detected excreting virus at the time of pregnancy checks, but fourteen coronavirus and two rotavirus carrier cows were found at parturition. All but two calves sampled had large numbers of virus particles in their feces. Clinical illness was associated with dams shedding virus and with nightly low temperatures.
在怀孕检查时和分娩时从两个肉牛群采集粪便样本。还在疾病发作时从患病犊牛采集样本,并在15日龄时从25%的健康犊牛采集样本。所有粪便样本均通过电子显微镜检查病毒。A群中有4头奶牛被检测出排泄冠状病毒,1头在怀孕检查时,3头在分娩时。第一头奶牛被从牛群中移出,其他奶牛在季节末产犊。没有患病犊牛。B群中在怀孕检查时未检测到排泄病毒的奶牛,但在分娩时发现了14头冠状病毒和2头轮状病毒携带奶牛。除2头采样犊牛外,所有犊牛粪便中都有大量病毒颗粒。临床疾病与排出病毒的母牛以及夜间低温有关。