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白蛋白掩盖了大鼠和人类血液蛋白质模式中的性别差异。

Albumin obscures sex differences in blood protein patterns of rats and humans.

作者信息

Gersten D M, Khirabadi B S, Kurian P, Ledley R S, Mahany T, Ramey E R, Ramwell P W

出版信息

Biochem J. 1980 Dec 1;191(3):869-72. doi: 10.1042/bj1910869.

DOI:10.1042/bj1910869
PMID:7283979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162290/
Abstract

Sex related differences in the blood protein patterns of male and female rats and humans have been studied by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. In rats, a prominent band of mol.wt. 74000--78000 is seen in females in far greater quantity than in males, castrated males or ovariectomized females. A secondary band of 100000 is seen under non-reducing conditions in female rats that is absent in males. In humans, bands of 92000 and 88000 mol.wt. appear to be variable in concentration in men although relatively constant in women. The above differences are observable only if serum albumin is removed from the samples before electrophoresis. The results suggest that each sex has its own characteristic blood protein pattern.

摘要

通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳研究了雄性和雌性大鼠及人类血液蛋白质模式的性别差异。在大鼠中,分子量为74000 - 78000的一条显著条带在雌性中出现的量远多于雄性、去势雄性或卵巢切除雌性。在非还原条件下,雌性大鼠中出现一条分子量为100000的次要条带,而雄性中不存在。在人类中,分子量为92000和88000的条带在男性中的浓度似乎可变,而在女性中相对恒定。上述差异只有在电泳前从样品中去除血清白蛋白时才可见。结果表明,每种性别都有其独特的血液蛋白质模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/d541eec3629a/biochemj00412-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/8e4ea4166c25/biochemj00412-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/79f7f81913e9/biochemj00412-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/8b8a50aa1faa/biochemj00412-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/a7292ad8ea96/biochemj00412-0202-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/d541eec3629a/biochemj00412-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/8e4ea4166c25/biochemj00412-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/79f7f81913e9/biochemj00412-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/8b8a50aa1faa/biochemj00412-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/a7292ad8ea96/biochemj00412-0202-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215f/1162290/d541eec3629a/biochemj00412-0203-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Women, work and coronary heart disease: prospective findings from the Framingham heart study.女性、工作与冠心病:弗明汉心脏研究的前瞻性研究结果
Am J Public Health. 1980 Feb;70(2):133-41. doi: 10.2105/ajph.70.2.133.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
High abundant protein removal from rodent blood for biomarker discovery.
从啮齿动物血液中去除高丰度蛋白质以发现生物标志物。
Biochem Biophys Res Commun. 2014 Dec 5;455(1-2):84-9. doi: 10.1016/j.bbrc.2014.09.137.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Selective removal of albumin from plasma by affinity chromatography.通过亲和色谱法从血浆中选择性去除白蛋白。
Clin Chim Acta. 1973 Nov 23;49(1):49-52. doi: 10.1016/0009-8981(73)90341-0.
5
New protein in human blood plasma, rich in proline, with lipid-binding properties.人血浆中的新蛋白质,富含脯氨酸,具有脂质结合特性。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1063-7. doi: 10.1073/pnas.73.4.1063.
6
Effects of a large dose of oestradiol on antithrombin III metabolism in male and female dogs.大剂量雌二醇对雄性和雌性犬抗凝血酶III代谢的影响。
Eur J Clin Invest. 1977 Oct;7(5):373-81. doi: 10.1111/j.1365-2362.1977.tb01622.x.
7
Cholesterol in the prediction of atherosclerotic disease. New perspectives based on the Framingham study.
Ann Intern Med. 1979 Jan;90(1):85-91. doi: 10.7326/0003-4819-90-1-85.
8
HDL cholesterol and other lipids in coronary heart disease. The cooperative lipoprotein phenotyping study.高密度脂蛋白胆固醇及其他脂质与冠心病。合作脂蛋白表型研究。
Circulation. 1977 May;55(5):767-72. doi: 10.1161/01.cir.55.5.767.
9
Studies and critique of Amido Black 10B, Coomassie Blue R, and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis.
Anal Biochem. 1979 Jul 15;96(2):263-78. doi: 10.1016/0003-2697(79)90581-5.
10
Two methods for the separation of human alpha-fetoprotein and albumin. (A) Affinity chromatography using Blue Sepharose CL-6B and (B) ampholyte displacement chromatography.两种分离人甲胎蛋白和白蛋白的方法。(A) 使用蓝色琼脂糖凝胶CL-6B的亲和色谱法和(B) 两性电解质置换色谱法。
Anal Biochem. 1978 Aug 1;88(2):619-23. doi: 10.1016/0003-2697(78)90464-5.