Natali A, Oxford J S, Schild G C
J Hyg (Lond). 1981 Oct;87(2):185-90. doi: 10.1017/s0022172400069394.
Antigenic variants of A/Texas/77 (H3N2) virus were selected in vitro using monoclonal antibody to virus haemagglutinin (HA). The antigenic variants and parental A/Texas/77 viruses were used to to evaluate the frequency of anti-HA antibodies in the sera of children and adults using single-radial-haemolysis (SRH) tests. Twenty to 41% of selected sera from adults, which contained antibody to the parental A/Texas/77 virus, failed to react with the different antigenic mutant viruses. A higher proportion of sera from children (37-58%) failed to react with the antigenic variants. Certain human sera and particularly those of children would appear to possess a more limited antibody repertoire to influenza HA, potentially allowing new antigenic variants to escape neutralization and spread in the community.
使用针对病毒血凝素（HA）的单克隆抗体在体外筛选出A/得克萨斯/77（H3N2）病毒的抗原变异体。利用单向辐射溶血（SRH）试验，用这些抗原变异体和亲本A/得克萨斯/77病毒来评估儿童和成人血清中抗HA抗体的频率。在含有针对亲本A/得克萨斯/77病毒抗体的成人所选血清中，20%至41%与不同的抗原突变病毒无反应。儿童血清中更高比例（37% - 58%）与抗原变异体无反应。某些人血清，尤其是儿童血清，似乎对流感HA的抗体谱更有限，这可能使新的抗原变异体逃避中和并在社区中传播。
