Peret J, Foustock S, Chanez M, Bois-Joyeux B, Robinson J L
J Nutr. 1981 Oct;111(10):1704-10. doi: 10.1093/jn/111.10.1704.
Changes in hepatic levels of lactate, pyruvate, phosphoenolpyruvate (PEP), alpha-ketoglutarate, malate, oxaloacetate, ketone bodies, alanine, serine, glycine, aspartate, glutamate, glutamine, valine, urea, adenine nucleotides and inorganic phosphate were examined in rats consuming a high protein, carbohydrate-free diet for up to 40 days. While some components showed transient changes, others (pyruvate, malate, oxaloacetate, PEP, ketone bodies, alanine, glycine, glutamine, valine, urea, adenine nucleotides and inorganic phosphate) were permanently altered. The cytoplasmic and mitochondrial redox states were only transiently affected and by day 24 were not different from control values. In contrast, the cytoplasmic phosphorylation state was affected from day 1 on; this suggests a role for the latter in permanently reorienting metabolism toward gluconeogenesis and ureogenesis.
对食用高蛋白、无碳水化合物饮食长达40天的大鼠肝脏中乳酸、丙酮酸、磷酸烯醇丙酮酸（PEP）、α-酮戊二酸、苹果酸、草酰乙酸、酮体、丙氨酸、丝氨酸、甘氨酸、天冬氨酸、谷氨酸、谷氨酰胺、缬氨酸、尿素、腺嘌呤核苷酸和无机磷酸盐水平的变化进行了检测。虽然一些成分显示出短暂变化，但其他成分（丙酮酸、苹果酸、草酰乙酸、PEP、酮体、丙氨酸、甘氨酸、谷氨酰胺、缬氨酸、尿素、腺嘌呤核苷酸和无机磷酸盐）发生了永久性改变。细胞质和线粒体的氧化还原状态仅受到短暂影响，到第24天时与对照值没有差异。相比之下，细胞质磷酸化状态从第1天起就受到影响；这表明后者在使代谢永久重新导向糖异生和尿素生成方面发挥了作用。
