Daszuta A, Barrit M C, Faudon M, Gambarelli F
J Physiol (Paris). 1981;77(2-3):315-8.
Adult C57BL and BALBc mice will exhibit differences in behaviour and in their sleep patterns which may be correlated with monoamine content. In this study, we measured the endogenous levels of serotonin (5-HT), tryptophan (TRP), 5-hydroxyindole acetic acid (5-HIAA), noradrenaline (NA) and dopamine (DA) in the forebrain and hindbrain regions of these strains of mice from day 1 to 12 weeks of age. Using the micropunch technique and a radioenzymatic method, we also determined the endogenous 5-HT levels in the nuclei raphe dorsalis, centralis, magnus-pallidus and obscurus. During development, NA levels in the brain stem are higher in the C57BL than in the BALBc mice. From 5 weeks of age onwards, the levels of 5-HT, DA and NA in the forebrain are also higher in the C57BL mice. However, no differences in 5-Ht and DA content was found for the brain stems of these two strains. When the 5-HT levels in the different raphe nuclei were analyzed, the nucleus raphe dorsalis of the BALBc showed a higher 5-HT content.
成年C57BL小鼠和BALBc小鼠在行为和睡眠模式上会表现出差异，这些差异可能与单胺含量相关。在本研究中，我们测量了这些品系小鼠从1日龄到12周龄期间前脑和后脑区域中血清素（5-羟色胺，5-HT）、色氨酸（TRP）、5-羟吲哚乙酸（5-HIAA）、去甲肾上腺素（NA）和多巴胺（DA）的内源性水平。使用微量冲压技术和放射酶法，我们还测定了中缝背核、中央核、大苍白核和中缝隐核中的内源性5-HT水平。在发育过程中，C57BL小鼠脑干中的NA水平高于BALBc小鼠。从5周龄起，C57BL小鼠前脑中的5-HT、DA和NA水平也更高。然而，这两个品系小鼠脑干中的5-Ht和DA含量没有差异。当分析不同中缝核中的5-HT水平时，BALBc小鼠的中缝背核显示出更高的5-HT含量。
