McLellan T, Ramshaw J A
Biochem Genet. 1981 Aug;19(7-8):647-54. doi: 10.1007/BF00483998.
We describe an electrophoretic method for the transfer of macromolecules from polyacrylamide slab gels to ion-exchange paper without loss of clarity or resolution. A series of partial transfers provides numerous copies of a single gel separation, and these copies may be assayed independently with enzyme specific stains. This method therefore combines the advantages of polyacrylamide gel electrophoresis for detecting enzyme variation with an efficient method for examining numerous enzymes from a single gel separation.
我们描述了一种电泳方法，可将大分子从聚丙烯酰胺平板凝胶转移至离子交换纸上，而不会损失清晰度或分辨率。一系列的部分转移可提供单个凝胶分离的多个副本，这些副本可用酶特异性染色剂独立检测。因此，该方法结合了聚丙烯酰胺凝胶电泳检测酶变异的优点以及从单个凝胶分离中检测多种酶的有效方法。
