生胃酮对大鼠胃上皮细胞中糖蛋白和DNA合成的影响。

Effect of carbenoxolone on the synthesis of glycoproteins and DNA in rat gastric epithelial cells.

作者信息

van Huis G A, Kramer M F

出版信息

Gut. 1981 Oct;22(10):782-7. doi: 10.1136/gut.22.10.782.

DOI:10.1136/gut.22.10.782

PMID:7297910

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1419420/

Abstract

The influence of carbenoxolone on the synthesis of glycoproteins in the surface mucous cells and the production of new cells in the rat gastric mucosa was studied by means of a vascular perfusion system. The rate of incorporation of tritiated galactose, glucosamine, serine, and sulphate in surface mucous cells, studied by autoradiography, was not affected by the addition of carbenoxolone to the drinking water. The sugar composition (determined by gas-liquid chromatography) of the gastric glycoproteins (isolated by centrifugation in CsCl), was not changed in carbenoxolone-treated rats. Compared with untreated animals, the number of [3H]-thymidine labelled nuclei per fundic pit increased by 38% to 76% in carbenoxolone-treated rats, implying a higher number of mitotically active cells. This results in an increased supply of young mucous cells; if this also proves to be true in human gastric mucosa, it may be relevant to the therapeutic effect of carbenoxolone.

摘要

通过血管灌注系统研究了甘珀酸对大鼠胃黏膜表面黏液细胞中糖蛋白合成及新细胞产生的影响。通过放射自显影术研究发现，向饮用水中添加甘珀酸并不影响表面黏液细胞中氚标记半乳糖、葡糖胺、丝氨酸和硫酸盐的掺入率。经甘珀酸处理的大鼠，通过氯化铯离心分离得到的胃糖蛋白的糖组成（通过气液色谱法测定）未发生变化。与未处理的动物相比，经甘珀酸处理的大鼠每个胃底小凹中[³H] - 胸腺嘧啶标记的细胞核数量增加了38%至76%，这意味着有丝分裂活跃细胞数量更多。这导致年轻黏液细胞的供应增加；如果在人类胃黏膜中也确实如此，那么这可能与甘珀酸的治疗效果相关。