Møller-Larsen A, Haahr S, Black F T
Infect Immun. 1978 Nov;22(2):445-51. doi: 10.1128/iai.22.2.445-451.1978.
A total of 17 children, aged 1 to 15 years, with gingivostomatitis were investigated to follow the development of immune parameters in those who suffered from herpes simplex virus stomatitis. Mouth swabs were obtained during the acute attack. Blood samples were collected on this occasion and again about 3 weeks later. Humoral immunity to herpes simplex virus was investigated by a complement fixation test and by an antibody-dependent cell-mediated cytotoxicity test. Cell-mediated immunity was investigated in a blast transformation assay with herpes simplex virus type 1 antigen and phytohemagglutinin. Interferon production in herpes-stimulated cultures was measured. Thirteen patients had a herpes simplex stomatitis. Twelve of these children were negative in the complement fixation test on the first serum specimen, but only five were negative in the antibody-dependent cell-mediated cytotoxicity test. These five were still febrile at the time of investigation. Blast transformation was negative at the first investigation in most children, whereas interferon was produced both in leukocyte cultures obtained during the infection and also in cultures made 3 to 4 weeks after the infection. An increase in immune parameters was seen in all patients with herpes stomatitis. From results in blast transformation and antibody-dependent cell-mediated cytotoxicity, it is seen that cell-mediated and humoral immunity can be found at the same time during recovery from this type of infection.
对17名年龄在1至15岁之间患龈口炎的儿童进行了调查，以追踪患单纯疱疹病毒口炎患儿免疫参数的变化情况。在急性发作期采集口腔拭子。此次采集血样，并在约3周后再次采集。通过补体结合试验和抗体依赖性细胞介导的细胞毒性试验研究对单纯疱疹病毒的体液免疫。通过用1型单纯疱疹病毒抗原和植物血凝素进行的淋巴细胞转化试验研究细胞介导免疫。测定疱疹刺激培养物中干扰素的产生。13名患者患有单纯疱疹性口炎。这些儿童中有12名在首次血清标本的补体结合试验中呈阴性，但在抗体依赖性细胞介导的细胞毒性试验中只有5名呈阴性。这五名儿童在调查时仍发热。大多数儿童在首次调查时淋巴细胞转化试验呈阴性，而在感染期间获得的白细胞培养物以及感染后3至4周进行的培养物中均产生了干扰素。所有患疱疹性口炎的患者免疫参数均有增加。从淋巴细胞转化试验和抗体依赖性细胞介导的细胞毒性试验结果可以看出，在这类感染的恢复过程中，细胞介导免疫和体液免疫可同时出现。
