Kono R, Sasagawa A, Yamazaki S, Nakazono N, Minami K, Otatsume S, Robin Y, Renaudet J, Cornet M, Afoakwa S N, Mingle J A, Obinim J K, Huros A
Am J Epidemiol. 1981 Sep;114(3):362-8. doi: 10.1093/oxfordjournals.aje.a113203.
One hundred and thirty-nine bovine sera collected in Senegal in 1968 and 1969, before the human pandemic of acute hemorrhagic conjunctivitis (AHC), and 145 sera collected in 1977, seven years after the introduction of AHC, were tested for virus neutralizing (VN) titers against enterovirus 70 (EV70) in neutralization tests. Positive rates of VN titers (1:16) were fairly constant (about 40%) in 1968 and 1969 but the proportion of positives from the 1977 collection was significantly higher (61%). The proportion of Ghanaian bovine sera positive in 1977 (39%) was comparable with those collected earlier in Senegal but the proportion was lower in sera of calves (7%). Similar studies were performed on sheep sera collected in 1966, 1967, 1968, and 1969 in Senegal. The proportions positive and the geometric mean titers (GMTs) peaked in alternate years: the proportions were 43% and 54% in 1966 and 1968 but dropped to 12% and 5% in 1967 and 1969, respectively. The prevalence rate for Ghanaian sheep sera in 1977 was comparable to the earlier figures in Senegal. The prevalence of VN (83%) and the GMT (1:27.28) in swine sera in Ghana in 1977 were much higher than those seen in chickens and dogs. However, 87 sera of wild monkeys caught in Senegal after 1970 were negative in neutralization tests. The VN substance detected in these domestic animals was found to be 2-mercaptoethanol sensitive; hence it is considered to belong to IgM. The results seem to favor the hypothesis that enterovirus type 70 (EV70) evolved from an animal enterovirus which shares a common antigen with EV70.
1968年和1969年在塞内加尔急性出血性结膜炎（AHC）大流行之前采集的139份牛血清，以及在AHC传入7年后的1977年采集的145份血清，在中和试验中检测了针对肠道病毒70（EV70）的病毒中和（VN）滴度。1968年和1969年，VN滴度（1:16）的阳性率相当稳定（约40%），但1977年采集的样本中阳性比例显著更高（61%）。1977年加纳牛血清的阳性比例（39%）与塞内加尔早些时候采集的血清相当，但犊牛血清中的比例较低（7%）。对1966年、1967年、1968年和1969年在塞内加尔采集的绵羊血清进行了类似研究。阳性比例和几何平均滴度（GMT）在交替年份达到峰值：1966年和1968年的比例分别为43%和54%，但在1967年和1969年分别降至12%和5%。1977年加纳绵羊血清的流行率与塞内加尔早期的数据相当。1977年加纳猪血清中VN的流行率（83%）和GMT（1:27.28）远高于鸡和狗。然而，1970年后在塞内加尔捕获的87份野生猴血清在中和试验中呈阴性。在这些家畜中检测到的VN物质对2-巯基乙醇敏感；因此，它被认为属于IgM。结果似乎支持肠道病毒70型（EV70）由与EV70具有共同抗原的动物肠道病毒进化而来的假说。
