Hierholzer J C, Bingham P G, Coombs R A, Stone Y O, Hatch M H
J Clin Microbiol. 1984 Jun;19(6):826-30. doi: 10.1128/jcm.19.6.826-830.1984.
We describe here a microneutralization procedure for conveniently testing large numbers of specimens for antibodies to enterovirus 70. The test utilized human rhabdomyosarcoma cells and was read by staining with crystal violet after 4 days of incubation. The test compares well with other serological assays, being more sensitive than the standard tube neutralization test and the complement fixation test, but less sensitive than the hemagglutination inhibition test. However, the hemagglutination inhibition test required concentrated, partially purified virus as antigen, as did the complement fixation test, and was difficult to read, so that its greater sensitivity may not be of practical significance. By all four test procedures, a recent isolate of enterovirus 70 was a more sensitive antigen than the prototype strain, as shown by greater geometric mean titers in sera of patients from various epidemics.
我们在此描述一种微量中和试验方法，用于方便地检测大量标本中的肠道病毒70抗体。该试验采用人横纹肌肉瘤细胞，孵育4天后用结晶紫染色进行判读。该试验与其他血清学检测方法相比效果良好，比标准试管中和试验和补体结合试验更敏感，但比血凝抑制试验敏感性稍低。然而，血凝抑制试验需要浓缩的部分纯化病毒作为抗原，补体结合试验也是如此，且判读困难，因此其更高的敏感性可能并无实际意义。通过所有四种检测方法，如来自不同疫情患者血清中更高的几何平均滴度所示，肠道病毒70的一株近期分离株作为抗原比原型株更敏感。
