蜡样芽孢杆菌β-内酰胺酶I催化头孢菌素水解的单周转和稳态动力学
Single-turnover and steady-state kinetics of hydrolysis of cephalosporins by beta-lactamase I from Bacillus cereus.
作者信息
Bicknell R, Waley S G
出版信息
Biochem J. 1985 Oct 1;231(1):83-8. doi: 10.1042/bj2310083.
Abstract
The kinetics of the hydrolysis of two cephalosporins by beta-lactamase I from Bacillus cereus 569/H/9 has been studied by single-turnover and steady-state methods. Single-turnover kinetics could be measured over the time scale of minutes when cephalosporin C was the substrate. The other substrate, 7-(2',4'-dinitrophenylamino)deacetoxycephalosporanic acid, was hydrolysed even more slowly, and has potential for use in crystallographic studies of beta-lactamases. Comparison of single-turnover and steady-state kinetics showed that, for both substrates, opening the beta-lactam ring (i.e. acylation of the enzyme) was the rate-determining step. Thus the non-covalent enzyme-substrate complex is expected to be the intermediate observed crystallographically.
摘要
利用单周转和稳态方法研究了蜡样芽孢杆菌569/H/9的β-内酰胺酶I对两种头孢菌素的水解动力学。以头孢菌素C为底物时，可在数分钟的时间尺度上测量单周转动力学。另一种底物7-(2',4'-二硝基苯氨基)去乙酰氧基头孢烷酸水解得更慢，有用于β-内酰胺酶晶体学研究的潜力。单周转和稳态动力学的比较表明，对于两种底物，β-内酰胺环的打开（即酶的酰化）是速率决定步骤。因此，非共价酶-底物复合物有望成为晶体学观察到的中间体。
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本文引用的文献
1
Proceedings of the biochemical society.生物化学学会会刊
Biochem J. 1966 Jan;98(1):1-16P.
2
Behaviour of some derivatives of 7-aminocephalosporanic acid and 6-aminopenicillanic acidas substrates, inhibitors and inducers of penicillinases.7-氨基头孢烷酸和6-氨基青霉烷酸的某些衍生物作为青霉素酶底物、抑制剂和诱导剂的行为。
Biochem J. 1962 Apr;83(1):52-63. doi: 10.1042/bj0830052.
3
A comparison of the action of penicillinase on benzylpenicillin and cephalosporin N and the competitive inhibition of penicillinase by cephalosporin C.青霉素酶对苄青霉素和头孢菌素N的作用以及头孢菌素C对青霉素酶的竞争性抑制作用的比较。
Biochem J. 1956 Aug;63(4):628-34. doi: 10.1042/bj0630628.
4
The ficin-catalysed hydrolysis of p-nitrophenyl hippurate. Detailed kinetics including the measurement of the apparent dissociation constant for the enzyme-substrate complex.无花果蛋白酶催化马尿酸对硝基苯酯的水解反应。详细的动力学，包括酶-底物复合物表观解离常数的测定。
FEBS Lett. 1969 Aug;4(4):299-306. doi: 10.1016/0014-5793(69)80261-9.
5
An easy method for the determination of initial rates.一种测定初始速率的简便方法。
Biochem J. 1981 Mar 1;193(3):1009-12. doi: 10.1042/bj1931009.
6
Half-time analysis of the integrated Michaelis equation. Simulation and use of the half-time plot and its direct linear variant in the analysis of some alpha-chymotrypsin, papain- and fumarase-catalysed reactions.米氏积分方程的半衰期分析。半衰期图及其直接线性变体在某些α-糜蛋白酶、木瓜蛋白酶和延胡索酸酶催化反应分析中的模拟与应用。
Biochem J. 1982 May 1;203(2):351-60. doi: 10.1042/bj2030351.
7
Inactivation of the RTEM beta-lactamase from Escherichia coli. Interaction of penam sulfones with enzyme.大肠杆菌中RTEMβ-内酰胺酶的失活。青霉烷砜与酶的相互作用。
Biochemistry. 1981 May 12;20(10):2726-31. doi: 10.1021/bi00513a004.
8
beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.β-内酰胺酶通过酰基酶中间体起作用。大肠杆菌RTEM酶与头孢西丁的相互作用。
Biochemistry. 1980 Jun 24;19(13):2895-901. doi: 10.1021/bi00554a012.
9
Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia.从嗜麦芽窄食单胞菌中分离出的诱导型青霉素β-内酰胺酶的纯化及特性
Antimicrob Agents Chemother. 1982 Oct;22(4):564-70. doi: 10.1128/AAC.22.4.564.
10
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