Coulombel C, Clermont S, Foglietti M J, Percheron F
Biochem J. 1981 Apr 1;195(1):333-5. doi: 10.1042/bj1950333.
By using [3H]mannobiose as a labelled acceptor, it was possible to demonstrate transfer reactions catalysed by two beta-mannanases, with mannotetraose and mannopentaose as substrates. The enzyme from Streptomyces transfers one mannose unit from the oligosaccharides, whereas the enzyme from fenugreek (Trigonella foenum-graecum) seeds is able to transfer oligomannose residues.
通过使用[3H]甘露二糖作为标记受体,得以证明两种β-甘露聚糖酶催化的转移反应,其底物分别为甘露四糖和甘露五糖。链霉菌来源的酶从寡糖上转移一个甘露糖单元,而胡芦巴(Trigonella foenum-graecum)种子来源的酶能够转移低聚甘露糖残基。