Sklov M C, Srinivasan S R, Webber L S, Berenson G S
Clin Chem. 1981 Dec;27(12):1988-92.
We evaluated the variability in total cholesterol concentrations in serum in a large population of children over a period of time, to help us discern the limitations in reliability of current clinical laboratory methods for its analysis. We analyzed sera from a population of approximately 4000 children over a four-year period, quality-control sera, pooled sera, and surveillance samples from the Centers for Disease Control over a seven-year period, with a Technicon AutoAnalyzer II. Two methods of correction were suggested to adjust deviations in yearly serum total cholesterol means: yearly screening data were corrected on the basis of monthly variability in pooled-serum determinations and on the basis of deviations from values suggested by the Centers for Disease Control. Both correction methods were insufficient. These changes occur very slowly, and unless there is close monitoring and frequent comparison with a known standard, changes in laboratory results still can occur.
我们评估了一大群儿童在一段时间内血清总胆固醇浓度的变异性，以帮助我们识别当前临床实验室分析方法在可靠性方面的局限性。我们在四年时间里分析了约4000名儿童的血清、质量控制血清、混合血清以及疾病控制中心在七年时间里的监测样本，使用的是Technicon AutoAnalyzer II。提出了两种校正方法来调整年度血清总胆固醇均值的偏差：年度筛查数据根据混合血清测定的月度变异性以及与疾病控制中心建议值的偏差进行校正。两种校正方法都不够充分。这些变化非常缓慢，除非进行密切监测并经常与已知标准进行比较，否则实验室结果仍可能出现变化。
