Slomiany B L, Kojima K, Banas-Gruszka Z, Murty V L, Galicki N I, Slomiany A
Eur J Biochem. 1981 Oct;119(3):647-50. doi: 10.1111/j.1432-1033.1981.tb05656.x.
A sulfated monosialosyltriglycosylceramide was isolated from the lipid extract of bovine gastric mucosa in a yield of 2.52 microM/kg of wet tissue. The isolation procedure involved extraction of lipids with 0.4 M sodium acetate in methanol/chloroform/water, alkaline methanolysis, column fractionation on DEAE-Sephadex and silicic acid, and thin-layer chromatography. Based on the results of partial acid hydrolysis, sequential degradation with specific glycosidases, periodate oxidation, and methylation analysis of the native and partially degraded compound, the structure of this glycolipid is proposed to be: GalNAc beta 1 leads to 4(SO3H leads to 8Sia alpha 2 leads to 3)Gal beta 1 leads to 4Glc1 leads to 1Cer. The sialic acid of this glycolipid consisted of N-acetylneuraminic acid and N-glycoloylneuraminic acid, with the former being predominant component (71%) of the mixture.
从牛胃黏膜脂质提取物中分离出一种硫酸化单唾液酸三糖神经酰胺，产量为每千克湿组织2.52微摩尔。分离过程包括用甲醇/氯仿/水混合液中的0.4 M醋酸钠提取脂质、碱性甲醇解、在DEAE-葡聚糖和硅酸上进行柱分级分离以及薄层色谱法。根据部分酸水解、用特定糖苷酶进行顺序降解、高碘酸盐氧化以及对天然和部分降解化合物的甲基化分析结果，推测这种糖脂的结构为：GalNAcβ1→4(SO3H→8Siaα2→3)Galβ1→4Glc1→1Cer。这种糖脂的唾液酸由N-乙酰神经氨酸和N-羟乙酰神经氨酸组成，前者是混合物中的主要成分（71%）。
