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猪葡萄球菌的分离与鉴定

Isolation and identification of Staphylococcus hyicus.

作者信息

Devriese L A

出版信息

Am J Vet Res. 1977 Jun;38(6):787-92.

PMID:879576
Abstract

A selective medium for the isolation of Staphylococcus hyicus, with potassium thiocyanate as selective substance and the polysorbate 80 reaction as elective marker, was developed. The organism could be identified during the routine examination of samples derived from pigs, using 2 relatively simple tests: the slide coagulase (clumping factor) and the deoxyribonuclease plate reaction. The S hyicus was isolated from 54% of 684 samples collected from nares, external ears, and skin covering the nose of pigs not affected by exudative epidermatitis. Isolates from these animals did not appear to differ in pathogenic potentials from those obtained from lesions.

摘要

开发了一种用于分离猪葡萄球菌的选择性培养基,以硫氰酸钾作为选择物质,以聚山梨醇酯80反应作为选择标记。在对来自猪的样本进行常规检查时,可通过2种相对简单的试验鉴定该菌:玻片凝固酶(凝聚因子)试验和脱氧核糖核酸酶平板反应。从684份取自未患渗出性皮炎的猪的鼻孔、外耳和鼻部皮肤的样本中,分离出猪葡萄球菌的比例为54%。这些动物的分离株在致病潜力方面似乎与从病变部位获得的分离株没有差异。

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