Wray C, Sojka W J
J Hyg (Lond). 1981 Dec;87(3):501-9. doi: 10.1017/s0022172400069758.
Small numbers of Salmonella dublin were used to infect calves in an attempt to simulate natural infection on the farm. Twenty calves were exposed to S. dublin by one or more of the following methods: Sucking cows which were excreting S. dublin in their faeces (less than 10(2)-10(5) organisms/g). Housing on S. dublin contaminated bedding. Drinking S. dublin contaminated water (10(2)-10(4) organisms/ml). During this experiment some calves were given therapeutic does of oxytetracycline. After exposure the calves were examined for faecal excretion of S. dublin (in some instances mouth swabs and blood samples were also examined) and for clinical signs of illness. Most of the calves became infected with S. dublin but excretion was usually sporadic and the numbers of salmonellas excreted were small. No clinical signs of salmonellosis were observed by S. dublin was isolated from one calf at post-mortem. Another six calves, dosed orally with either 10(6) or 10(8) S. dublin, showed signs of mild illness and although three calves had diarrhoea excretion of salmonellas was intermittent. S. dublin was isolated from one of these calves at post-mortem.
使用少量都柏林沙门氏菌感染犊牛，试图模拟农场中的自然感染情况。20头犊牛通过以下一种或多种方法接触都柏林沙门氏菌：吸吮粪便中排泄都柏林沙门氏菌的母牛（每克粪便中细菌数量少于10² - 10⁵个）；饲养在被都柏林沙门氏菌污染的垫料上；饮用被都柏林沙门氏菌污染的水（每毫升含10² - 10⁴个细菌）。在该实验过程中，一些犊牛接受了治疗剂量的土霉素。接触后，检查犊牛粪便中都柏林沙门氏菌的排泄情况（某些情况下还检查口腔拭子和血液样本）以及疾病的临床症状。大多数犊牛感染了都柏林沙门氏菌，但排泄通常是零星的，排出的沙门氏菌数量很少。未观察到沙门氏菌病的临床症状，但在一头犊牛死后分离出了都柏林沙门氏菌。另外6头口服10⁶或10⁸个都柏林沙门氏菌的犊牛出现了轻度疾病症状，尽管3头犊牛腹泻，但沙门氏菌的排泄是间歇性的。在其中一头犊牛死后分离出了都柏林沙门氏菌。
