Fisher S E, Atkinson M, Holzman I, David R, Van Thiel D H
Prog Biochem Pharmacol. 1981;18:216-23.
The effect of ethanol infusion upon placental uptake of amino acids was studied in pregnant sheep. Blood ethanol levels of 150-260 mg/dl obliterated the normal uptake of amino acids by the in vivo placenta. However, when human placental villi were incubated in vitro with ethanol at 300 mg/dl, there was no inhibition of uptake of 14C alpha-amino isobutyric acid (AIB). In contrast, 2-20 mM, but not 50 or 200 micro M, acetaldehyde significantly inhibited AIB uptake (% inhibition at 90 min: 2 mM, 23.5 +/- 9.6%; 5 mM, 33.8 +/- 4.7%; 10 mM, 54.6 +/- 2.9%; 20 mM, 61.5 +/- 7.5%; p less than 0.01 for all 4 concentrations). When human placental villi were preincubated with 10 micro M acetaldehyde, washed, and incubated with 14C-AIB in the absence of acetaldehyde, there was significant residual inhibition of AIB uptake. The data suggest that ethanol-associated placental injury may contribute to the pathophysiology of the fetal alcohol syndrome.
在怀孕母羊中研究了乙醇输注对胎盘摄取氨基酸的影响。血液乙醇水平为150 - 260mg/dl时，会消除体内胎盘对氨基酸的正常摄取。然而，当人胎盘绒毛在体外与300mg/dl乙醇孵育时，对14Cα-氨基异丁酸（AIB）的摄取没有抑制作用。相反，2 - 20mM而非50或200μM的乙醛显著抑制AIB摄取（90分钟时的抑制百分比：2mM，23.5±9.6%；5mM，33.8±4.7%；10mM，54.6±2.9%；20mM，61.5±7.5%；所有4种浓度p均小于0.01）。当人胎盘绒毛先用10μM乙醛预孵育、洗涤，然后在无乙醛的情况下与14C - AIB孵育时，对AIB摄取有显著的残留抑制作用。数据表明，与乙醇相关的胎盘损伤可能导致胎儿酒精综合征的病理生理学改变。
