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[采用自动方法进行的细菌鉴定试验和抗生素敏感性试验的可靠性]

[Reliability of the bacterial identification tests and of antibiotic sensitivity tests made with automatic methods].

作者信息

Goffi M, Somenzi P, Magliani W, Menozzi M G

出版信息

Quad Sclavo Diagn. 1981 Mar;17(1):61-72.

PMID:7313071
Abstract

A "MS-2 Microbiology System" (Abbott) has been used to identify and to assess the susceptibility patterns of clinically isolated bacteria from highly compromised patients. Automatically obtained results by the analysis of 300 specimens were compared to those of traditional methods. The automated system was in good agreement with API (81%) while in 4% of the strains there were major discrepancies, and 5% of the experiments were carried out with slow growing bacteria which normally give poor results in automatic systems. Antimicrobial susceptibility testing was satisfactory in 84% of the specimens with 7% of minor discrepancies and 9% of major ones. The evaluation of MIC for slow growing organisms like Haemophilus, Diplococcus and anaerobes belonging to the Bacteroides genus, was the same using either automatic or standard procedures.

摘要

已使用“MS-2微生物系统”(雅培公司)来鉴定和评估从重症患者临床分离出的细菌的药敏模式。将通过分析300份标本自动获得的结果与传统方法的结果进行比较。该自动化系统与API的一致性良好(81%),而在4%的菌株中存在重大差异,并且5%的实验针对生长缓慢的细菌进行,这类细菌在自动化系统中通常结果不佳。84%的标本的药敏试验结果令人满意,其中7%存在轻微差异,9%存在重大差异。对于生长缓慢的微生物,如嗜血杆菌、双球菌以及类杆菌属厌氧菌,使用自动程序或标准程序评估的MIC相同。

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