Sehulster L M, Hollinger F B, Dreesman G R, Melnick J L
Appl Environ Microbiol. 1981 Nov;42(5):762-7. doi: 10.1128/aem.42.5.762-767.1981.
Sodium hypochlorite (NaOCl) was examined as an effective disinfectant in hepatitis laboratories. Concentrations of NaOCl containing 5,600 ppm (5,600 microgram/ml) of available chlorine were found to be effective in destroying the antigenicity of hepatitis B surface antigen (HBsAg) in virion-rich plasma after an exposure time of 1 min or more. In the treatment of protein-deficient solutions containing HBsAg, smaller concentrations of available chlorine (less than 500 pm) are equally effective. Neither 17-to 25-nm HBsAg particles nor 45-nm virion particles could be detected by electron microscopy after treatment. chemical interaction of protein and NaOCl was confirmed by isoelectrofocusing of 125I-labeled HBsAg. More than 90% of the labeled material was found at pH 3.0 or lower, indicating complete antigen oxidation. Labeled HBsAg was reduced in density from 1.21 g/cm3 in CsCl to approximately 1.07 g/cm3 after treatment with NaOCl. Both hepatitis B core antigen and deoxyribonucleic acid polymerase activity were significantly reduced after interaction with hypochlorite solutions. These results show that NaOCl destroys hepatitis B antigenicity and virus structures and therefore may be utilized as a disinfectant for the virus.
次氯酸钠（NaOCl）在肝炎实验室中被作为一种有效的消毒剂进行了检测。发现含有5600 ppm（5600微克/毫升）有效氯的次氯酸钠溶液，在暴露1分钟或更长时间后，能有效破坏富含病毒粒子的血浆中乙肝表面抗原（HBsAg）的抗原性。在处理含有HBsAg的蛋白质缺乏溶液时，较低浓度的有效氯（低于500 ppm）同样有效。处理后通过电子显微镜未检测到17至25纳米的HBsAg颗粒或45纳米的病毒粒子。通过对125I标记的HBsAg进行等电聚焦证实了蛋白质与次氯酸钠的化学相互作用。发现超过90%的标记物质在pH 3.0或更低时出现，表明抗原完全被氧化。用次氯酸钠处理后，标记的HBsAg在CsCl中的密度从1.21克/立方厘米降至约1.07克/立方厘米。与次氯酸盐溶液相互作用后，乙肝核心抗原和脱氧核糖核酸聚合酶活性均显著降低。这些结果表明，次氯酸钠可破坏乙肝抗原性和病毒结构，因此可作为该病毒的消毒剂。
