氯乙烯代谢物硫代二甘醇酸的色谱测定

Chromatographic determination of thiodiglycolic acid - a metabolite of vinyl chloride.

作者信息

Dramiński W, Trojanowska B

出版信息

Arch Toxicol. 1981 Nov;48(4):289-92. doi: 10.1007/BF00319657.

DOI:10.1007/BF00319657

Abstract

The determination of thiodiglycolic acid (TDGA) In urine by gas chromatography (GC) is described. The analytical procedure includes addition of internal standard (o-phthalic acid), ethyl acetate extraction, evaporation of the solvent and silylation of TDGA with N-trimethylsilyl-diethylamine in pyridine (1:1). The results are calculated from the ratio of TDGA and internal standard peak heights. The detection limit of the method is 10 mg/l and the coefficient of various is +/- 5%.

摘要

本文描述了采用气相色谱法（GC）测定尿液中的硫代二甘醇酸（TDGA）。分析步骤包括加入内标（邻苯二甲酸）、用乙酸乙酯萃取、溶剂蒸发以及在吡啶（1:1）中用N-三甲基甲硅烷基二乙胺对TDGA进行硅烷化。结果根据TDGA与内标峰高之比计算得出。该方法的检测限为10 mg/l，变异系数为±5%。