Iversen O H, Thorud E, Volden G
Carcinogenesis. 1981;2(11):1129-33. doi: 10.1093/carcin/2.11.1129.
Hairless mice were given 5 topical applications of 470 nmol 20-methylcholanthrene (MCA) at one week intervals. In one experimental group the MCA was dissolved in reagent grade acetone alone, in another group it was dissolved in a mixture consisting of 50% acetone and 50% dimethyl sulfoxide (DMSO). A control group received the mixed solvent alone. The animals were observed for development of papillomas and malignant tumors during 75 weeks. The group treated with MCA in acetone/DMSO had a higher mortality than the two other groups. The admixture of 50% DMSO to the solvent had a significant inhibitory effect on tumor and cancer rates and on cancer yield, whereas no effect could be observed on the tumor yield. Hence, 50% DMSO in the solvent has a moderate, but significant, inhibitor effect on MCA-induced skin carcinogenesis. Similar inhibitory effect of DMSO on the promotion phase in two stage-carcinogenesis protocols have been reported in the literature. The biochemical mechanisms behind this effect are unknown.
无毛小鼠每隔一周接受5次局部涂抹470纳摩尔的20-甲基胆蒽（MCA）。在一个实验组中，MCA仅溶解于试剂级丙酮中，在另一组中，它溶解于由50%丙酮和50%二甲基亚砜（DMSO）组成的混合物中。一个对照组仅接受混合溶剂。在75周内观察动物乳头状瘤和恶性肿瘤的发生情况。用丙酮/二甲基亚砜中的MCA处理的组比其他两组死亡率更高。溶剂中50%DMSO的混合物对肿瘤和癌症发生率以及癌症产量有显著的抑制作用，而对肿瘤产量未观察到影响。因此，溶剂中50%DMSO对MCA诱导的皮肤致癌作用有适度但显著的抑制作用。文献中已报道DMSO在两阶段致癌方案中对促进阶段有类似的抑制作用。这种作用背后的生化机制尚不清楚。
