Pernice W, Sodomann C P, Lüben G, Seiler F R, Sedlacek H H
Clin Exp Immunol. 1979 Aug;37(2):376-80.
In recent studies extrahepatic manifestations of viral hepatitis have been recognized as immune complex diseases. Hepatitis B surface antigen (HBsAg) has been successfully identified in immune complexes, but the pathogenic role of HBsAg-containing immune complexes (IC) remains questionable. The subject of the present study was the antigen-specific determination of IC in the course of hepatitis B virus infection using a new HBsAg-specific IC test (Pernice & Sedlacek, 1978). This test is based on the following principle: rabbit anti-HBs-coated polystyrole test tubes are incubated with the IC-containing test sample. The HBsAg-containing IC bind to the solid phase by their free antigenic determinants. There they can be quantified using a peroxidase-labelled anti-human IgG antibody. A good correlation was found between the level of HBsAg-containing immune complexes and the clinical state of six patients in a follow-up study. IC could be detected simultaneously with HBsAg and either decreased or disappeared before the occurrence of free anti-HBs. In the sera of an additional twenty-eight patient suffering from chronic active hepatitis, HBsAg-containing immune complexes were detected in 85% of cases. One patient suffering from polyarteritis nodosa was also positive. Occasionally, extremely high levels of IC were found in the course of these diseases.
在最近的研究中，病毒性肝炎的肝外表现已被确认为免疫复合物疾病。乙肝表面抗原（HBsAg）已在免疫复合物中成功鉴定出来，但含HBsAg的免疫复合物（IC）的致病作用仍存在疑问。本研究的主题是使用一种新的HBsAg特异性IC检测方法（Pernice和Sedlacek，1978年），对乙肝病毒感染过程中的IC进行抗原特异性测定。该检测基于以下原理：将兔抗HBs包被的聚苯乙烯试管与含IC的检测样品一起孵育。含HBsAg的IC通过其游离抗原决定簇与固相结合。在那里，可以使用过氧化物酶标记的抗人IgG抗体对其进行定量。在一项随访研究中，发现含HBsAg的免疫复合物水平与6名患者的临床状态之间存在良好的相关性。IC可以与HBsAg同时检测到，并且在游离抗HBs出现之前降低或消失。在另外28例慢性活动性肝炎患者的血清中，85%的病例检测到含HBsAg的免疫复合物。1例结节性多动脉炎患者也呈阳性。在这些疾病过程中偶尔会发现极高水平的IC。
