de la Torre B, Sjöberg B, Hedman M, Bártfai G, Diczfalusy E
Int J Androl. 1981 Oct;4(5):532-45. doi: 10.1111/j.1365-2605.1981.tb00736.x.
Using radioimmunoassay method, we have estimated the levels of cortisol (C), pregnenolone (delta 5P), 17-hydroxypregnenolone (17-OH-delta 5P), dehydroepiandrosterone (DHEA), 17-hydroxyprogesterone (17-OH-P), androstenedione (A), testosterone (T) and dihydrotestosterone (DHT) in peripheral plasma samples collected at short-time (15 min) intervals from 10 fertile men, during two 4-h periods (06.00 to 10.00 and 18.00 to 22.00). In addition, the levels of biologically active luteinizing hormone (LH) were measured by an in vitro bioassay method in 9 of the subjects. The levels of all steroids studied exhibited diurnal variation with higher levels during the morning and lower levels during the evening period. The cortisol and the delta 5-steroid levels also exhibited individual short-term episodic spikes during the 2 periods. No short-term variation was observed in the levels of 17-OH-P, A, T and DHT. Statistically significant correlations were found between the levels of C and those of the delta 5-steroids and A in most of the subjects. No correlation was found between the above steroid levels and those of 17-OH-P, T and DHT. Also the LH levels exhibited episodic spikes of 60 to 90 min duration, but no diurnal variation. When the LH levels were related to those of T found in the same samples or in samples withdrawn 15 to 810 min afterwards, a significant positive correlation was found on repeated occasions in 5 of the 9 subjects. No systematic negative correlation was found when the T levels were related to those of LH in the same sequential fashion. Whereas the positive correlations found between LH and T levels in some of the subjects might suggest that physiological changes in peripheral LH levels are instrumental in regulating T-secretion, the rather consistent lack of significant negative correlation between T and LH levels seems to favour the view that the release of LH is not modulated by peripheral testosterone levels alone.
采用放射免疫分析法，我们测定了10名生育期男性在两个4小时时间段（06:00至10:00和18:00至22:00）内每隔15分钟采集的外周血样本中皮质醇（C）、孕烯醇酮（δ5P）、17-羟孕烯醇酮（17-OH-δ5P）、脱氢表雄酮（DHEA）、17-羟孕酮（17-OH-P）、雄烯二酮（A）、睾酮（T）和双氢睾酮（DHT）的水平。此外，采用体外生物测定法测定了9名受试者中生物活性促黄体生成素（LH）的水平。所研究的所有类固醇水平均呈现昼夜变化，上午水平较高，傍晚水平较低。在这两个时间段内，皮质醇和δ5-类固醇水平还出现了个体短期的突发性峰值。17-OH-P、A、T和DHT水平未观察到短期变化。在大多数受试者中，发现C水平与δ5-类固醇和A水平之间存在统计学显著相关性。上述类固醇水平与17-OH-P、T和DHT水平之间未发现相关性。LH水平也出现了持续60至90分钟的突发性峰值，但无昼夜变化。当将LH水平与同一样本或之后15至810分钟采集的样本中的T水平相关联时，在9名受试者中的5名中多次发现显著正相关。当以相同顺序将T水平与LH水平相关联时，未发现系统性负相关。虽然在一些受试者中发现LH和T水平之间的正相关可能表明外周LH水平的生理变化有助于调节T分泌，但T和LH水平之间相当一致地缺乏显著负相关似乎支持这样一种观点，即LH的释放并非仅由外周睾酮水平调节。
