Redmond O, Tuffery A R
J Anat. 1981 Aug;133(Pt 1):37-47.
Male rats (200 g body weight) were either untreated or given carbimazole (0.1 g/100 mg) in their drinking water for up to 24 weeks. Vincristine sulphate - a metaphase arrest agent - was used to estimate the mitotic activity of thyroid follicular cells. Measurements of body growth rate and oxygen consumption showed that the animals were hypothyroid after one week of treatment. The relationship between body growth and mitotic rate supports the concept of the thyroid follicular cells as an 'expanding' cell population. The mitotic rate (metaphases/10(4) cells . hour +/- S.E.) rose from the control level of 3.9 +/- 1.28 to 33.3 +/- 7.59 after 2 days of treatment and reached a peak of 79.9 +/- 8.56 on day 5. Thereafter, it declined slowly, but remained significantly greater than control levels until the 16th week of treatment. Changes in mitotic activity were accompanied by characteristic histological changes in the gland. After two days' treatment with carbimazole, the serum concentration of thyrotropin (TSH) was significantly increased and the serum levels of tri- and tetra-iodothyronine were decreased. These results area compared with those of earlier studies and discussed in relation to changes in hormone levels and strongly suggest that TSH is responsible for the hyperplasia of thyroid follicular cells to respond to continued stimulation.
雄性大鼠（体重200克）分为未处理组和在饮用水中给予卡比马唑（0.1克/100毫克）长达24周的处理组。硫酸长春新碱（一种中期阻断剂）用于评估甲状腺滤泡细胞的有丝分裂活性。对身体生长速率和耗氧量的测量表明，处理一周后动物出现甲状腺功能减退。身体生长与有丝分裂率之间的关系支持了甲状腺滤泡细胞作为“增殖性”细胞群体的概念。有丝分裂率（中期/10⁴细胞·小时±标准误）在处理2天后从对照水平的3.9±1.28升至33.3±7.59，并在第5天达到峰值79.9±8.56。此后，它缓慢下降，但直到处理第16周仍显著高于对照水平。有丝分裂活性的变化伴随着腺体特征性的组织学变化。用卡比马唑处理两天后，促甲状腺激素（TSH）的血清浓度显著升高，三碘甲状腺原氨酸和四碘甲状腺原氨酸的血清水平降低。这些结果与早期研究的结果进行了比较，并结合激素水平的变化进行了讨论，强烈表明TSH负责甲状腺滤泡细胞的增生以应对持续刺激。
