Starov A I, Bukrinskaia A G
Vopr Virusol. 1980 Sep-Oct(5):611-5.
An analysis of poly A(+)-and poly A(--)-transcripts in influenza A/WSN (H0N1) virus-infected cells done by the method of Hay and coworkers demonstrated three stages of transcription: (1) primary transcription during which mRNA of all the genes is synthesized, (2) early secondary transcription during which mRNA of NS gene is amplified, and (3) late secondary transcription in which mRNA of all the genes is amplified. Poly A(--) transcripts are synthesized at the stage of secondary transcription. The effect of three concentrations of actinomycin D at various stages of transcription was studied. The experimental results suggest differences in inhibition by actinomycin D of synthesis of transcripts of both types at the early and late stages of secondary transcription. The effect on the infected cells of alpha-amanitine specifically inhibiting synthesis of primary mRNA showed the synthesis of cellular RNAs to be much more necessary for the synthesis of polyA(+)-transcripts than for that of polyA(--)-transcripts.
用海伊及其同事的方法对感染甲型流感病毒/WSN（H0N1）的细胞中的多聚腺苷酸（+）和多聚腺苷酸（-）转录本进行分析，结果表明转录过程分为三个阶段：（1）初级转录阶段，在此期间合成所有基因的信使核糖核酸；（2）早期次级转录阶段，在此期间NS基因的信使核糖核酸被扩增；（3）晚期次级转录阶段，在此期间所有基因的信使核糖核酸被扩增。多聚腺苷酸（-）转录本在次级转录阶段合成。研究了三种浓度的放线菌素D在转录不同阶段的作用。实验结果表明，在次级转录的早期和晚期，放线菌素D对两种类型转录本合成的抑制作用存在差异。特异性抑制初级信使核糖核酸合成的α-鹅膏蕈碱对感染细胞的作用表明，细胞核糖核酸的合成对于多聚腺苷酸（+）转录本的合成比对于多聚腺苷酸（-）转录本的合成更为必要。
