Sbrana G, Migliorini M G, Vecchi C, Donzelli G P
Pediatr Res. 1981 Dec;15(12):1517-9. doi: 10.1203/00006450-198112000-00013.
Photodegradation of bilirubin in vitro has been investigated by using monochromatic light supplied by an argon ion laser selecting the 457.9, 488.0 and 514.5 nm wavelengths. Bilirubin was examined in chloroform, in aqueous solutions and in human serum under different experimental conditions of concentration, laser power and time of irradiance. Photodecomposition was followed by optical density measurements on the absorption maximum of the electronic band at 460 nm. The rate of degradation of bilirubin was found to be only slightly affected by the wavelength of the exciting lines provided they fall within the absorption band. In particular it was shown that any wavelength, lambda, is equally effective if the corresponding absorbance, A lambda, exceeds a minimum value of 5-10%. In the aqueous solutions, light with lambda greater than 470 nm has been found to be largely effective in the photodegradation of bilirubin in vitro.
通过使用氩离子激光提供的单色光，选择457.9、488.0和514.5纳米波长，对胆红素的体外光降解进行了研究。在不同的浓度、激光功率和辐照时间实验条件下，对氯仿、水溶液和人血清中的胆红素进行了检测。通过对460纳米电子带吸收最大值的光密度测量来跟踪光分解过程。发现只要激发线的波长落在吸收带内，胆红素的降解速率仅受其轻微影响。特别是研究表明，如果相应的吸光度Aλ超过5 - 10%的最小值，任何波长λ都是同样有效的。在水溶液中，已发现波长大于470纳米的光在体外对胆红素的光降解非常有效。
