Riou G, Benard J
Nouv Presse Med. 1981 Dec 5;10(44):3643-6.
Tumoral stem cells capable of multiplying can be selected from human tumours by in vitro culture techniques. These are the cells which renew the tumour and form metastases. Placed in a suitable agar medium, they give birth to cellular clones. The selection of clone-forming cells from human tumours makes it possible to devise chemograms, as has successfully been done in the U.S.A. by the Salmon and von Hoff teams. Working on a fairly large number of different cancers and using in vitro tests in the presence of drugs, these scientists were able to predict chemosensitivity and chemoresistance in 62 to 96% of tumours. The new techniques and results are presented and discussed.
能够增殖的肿瘤干细胞可通过体外培养技术从人类肿瘤中筛选出来。这些细胞能够使肿瘤更新并形成转移灶。将它们置于合适的琼脂培养基中，会产生细胞克隆。从人类肿瘤中筛选出克隆形成细胞使得制定化疗图谱成为可能，美国的萨尔蒙和冯·霍夫团队就成功做到了这一点。这些科学家研究了相当多不同类型的癌症，并在有药物存在的情况下进行体外测试，从而能够在62%至96%的肿瘤中预测化疗敏感性和化疗耐药性。本文展示并讨论了这些新技术和研究结果。
