胆固醇喂养对兔胆囊胆汁酸的影响。证明石胆酸是兔的一种初级胆汁酸。
The effect of cholesterol feeding on gallbladder bile acids of the rabbit. Evidence that lithocholic acid is a primary bile acid in the rabbit.
作者信息
Taylor W, Ellis W R, Bell G D
出版信息
Biochem J. 1981 Sep 15;198(3):639-43. doi: 10.1042/bj1980639.
The bile acid in gallbladder bile of rabbits fed a normal diet or one containing 2% (w/w) cholesterol have been determined by gas chromatography-mass spectrometry. The predominant bile acids in normally fed rabbits were 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholan-24-oic acid (cholic acid), 3 alpha, 12 alpha-dihydroxy-5 alpha-cholan-24-oic acid (allodeoxycholic acid) and 3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oic acid (deoxycholic acid) with very much smaller amounts of 3 alpha-hydroxy-5 beta-cholan-24-oic acid (lithocholic acid) and 3 alpha, 12 beta-dihydroxy-5 beta-cholan-24-oic acid. In the cholesterol-fed animals the lithocholate became a predominant bile acid. Sulphated bile acids accounted for less than 1% of the total bile acids. It is proposed that lithocholic acid may be a primary bile acid in the cholesterol-fed rabbit, formed by an alternative pathway of biosynthesis involving hepatic mitochondria.
采用气相色谱 - 质谱联用技术测定了正常饮食或含2%（w/w）胆固醇饮食的家兔胆囊胆汁中的胆汁酸。正常饮食家兔中主要的胆汁酸为3α，7α，12α - 三羟基 - 5β - 胆烷 - 24 - 酸（胆酸）、3α，12α - 二羟基 - 5α - 胆烷 - 24 - 酸（别脱氧胆酸）和3α，12α - 二羟基 - 5β - 胆烷 - 24 - 酸（脱氧胆酸），而3α - 羟基 - 5β - 胆烷 - 24 - 酸（石胆酸）和3α，12β - 二羟基 - 5β - 胆烷 - 24 - 酸的含量极少。在喂食胆固醇的动物中，石胆酸盐成为主要的胆汁酸。硫酸化胆汁酸占总胆汁酸的比例不到1%。有人提出，石胆酸可能是喂食胆固醇的家兔中的一种初级胆汁酸，它通过涉及肝线粒体的另一条生物合成途径形成。
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