Cattell V, Arlidge S
Br J Exp Pathol. 1981 Dec;62(6):669-75.
The origin of proliferating cells in glomeruli and Bowman's capsules during nephrotoxic nephritis (NTN) in rabbits was studied, using unilateral renal irradiation to suppress glomerular cell division. Left (LI) kidneys received 2400-12,000 rad on Days 1-5 after i.v. nephrotoxic serum (NTS) 1 or 1.5 ml/kg body wt. Renal histology, cell labelling 1 h after a pulse of tritiated thymidine (H3T) (0.5 mCi/kg), and mitotic rate were compared in right non-irradiated (RO) kidneys and LI kidneys. Differences in H3T uptake were not significantly suppressed (P less than 0.05) by 4800 rad whereas mitoses in glomeruli and Bowman's capsule were not. Glomerular hypercellularity and crescents were equivalent in LI given 4800 rad and in RO kidneys, but fibrin deposition was increased 2-fold in LI kidneys. In control rabbits (no NTS) LI kidneys showed no histological changes. The results suggest that most dividing cells detected in glomeruli and Bowman's space in NTN are monocytes, and that these cells form a large component of crescents.
采用单侧肾脏照射抑制肾小球细胞分裂，对兔肾毒性肾炎（NTN）期间肾小球和鲍曼囊内增殖细胞的来源进行了研究。在静脉注射1或1.5 ml/kg体重的肾毒性血清（NTS）后第1至5天，左侧（LI）肾脏接受2400 - 12000拉德的照射。对右侧未照射（RO）肾脏和LI肾脏的肾组织学、氚标记胸腺嘧啶核苷（H3T）（0.5 mCi/kg）脉冲标记1小时后的细胞标记情况以及有丝分裂率进行了比较。4800拉德的照射并未显著抑制（P < 0.05）H3T摄取，但肾小球和鲍曼囊内的有丝分裂受到抑制。接受4800拉德照射的LI肾脏和RO肾脏中的肾小球细胞增多和新月体形成情况相当，但LI肾脏中的纤维蛋白沉积增加了2倍。在对照兔（未注射NTS）中，LI肾脏未显示组织学变化。结果表明，在NTN中肾小球和鲍曼间隙中检测到的大多数分裂细胞是单核细胞，并且这些细胞构成了新月体的很大一部分。
