Farbman A I, Telser A, Chacko C
Histochemistry. 1981 Dec;73(3):419-28. doi: 10.1007/BF00495656.
A low molecular weight (LMW) protein was isolated from bovine tongue epithelium and an antiserum to this protein elicited in rabbits. The indirect peroxidase-antiperoxidase (PAP) technique was used to localize LMW protein in several tissues from six mammalian species: cow, rat, mouse, squirrel, rabbit, and man. Immunoreactivity was demonstrable in stratified squamous epithelia from skin, tongue, cheek, esophagus, vagina, and palate. Epidermal derivatives, such as hair follicles, sebaceous glands and ducts of certain glands were also positively stained. Cornea exhibited weak immunoreactivity as did rabbit bladder. Other types of epithelia including those seen in kidney, thyroid, intestine, trachea, liver, submandibular gland, pancreas and uterus, were not immunoreactive when tested with antiserum to LMW protein. The antiserum was rendered unreactive after absorption with LMW protein but, when absorbed with a keratin polypeptide, most of the immunoreactivity was preserved. It is concluded that the distribution of the soluble LMW protein is similar to that of the insoluble keratin proteins in stratified squamous epithelia but the former is not demonstrable in many simple epithelia that contain keratins.
从牛舌上皮中分离出一种低分子量（LMW）蛋白质，并在兔体内制备了针对该蛋白质的抗血清。采用间接过氧化物酶-抗过氧化物酶（PAP）技术，在六种哺乳动物（牛、大鼠、小鼠、松鼠、兔和人）的多种组织中定位LMW蛋白质。在皮肤、舌、颊、食管、阴道和腭的复层鳞状上皮中可检测到免疫反应性。表皮衍生物，如毛囊、皮脂腺和某些腺体的导管也呈阳性染色。角膜和兔膀胱一样表现出较弱的免疫反应性。用针对LMW蛋白质的抗血清检测时，包括肾、甲状腺、肠、气管、肝、下颌下腺、胰腺和子宫中的其他类型上皮均无免疫反应性。抗血清用LMW蛋白质吸收后失去反应性，但用角蛋白多肽吸收时，大部分免疫反应性得以保留。结论是，可溶性LMW蛋白质的分布与复层鳞状上皮中不溶性角蛋白的分布相似，但前者在许多含角蛋白的单层上皮中无法检测到。
