Fedak M A, Rome L, Seeherman H J
J Appl Physiol Respir Environ Exerc Physiol. 1981 Sep;51(3):772-6. doi: 10.1152/jappl.1981.51.3.772.
A simple one-step procedure that eliminates the need to calibrate the O2 analyzer or measure the flow past the animal is described for calibrating an open-flow respirometry system. The technique is particularly useful for situations of high ambient humidity and for large or active animals where a mask is employed to capture expired gases. A measured N2 flow is used to calibrate the system. The equations describing the technique are given, and the accuracy of the method is discussed in detail. The errors associated with the technique are compared with those of more conventional procedures and are usually smaller.
本文描述了一种简单的一步法，用于校准开放流动式呼吸测量系统，该方法无需校准氧气分析仪或测量流经动物的气流。该技术对于高环境湿度的情况以及使用面罩收集呼出气体的大型或活跃动物尤为有用。使用测量的氮气流量来校准系统。给出了描述该技术的方程式，并详细讨论了该方法的准确性。将该技术相关的误差与更传统方法的误差进行了比较，结果表明该技术的误差通常较小。
