Mettrick D F, Rahman M S, Podesta R B
Mol Biochem Parasitol. 1981 Dec;4(3-4):217-23. doi: 10.1016/0166-6851(81)90020-7.
Glucose uptake by Hymenolepis diminuta was linear for up to 60 min following in vitro incubation in 10 mM glucose. Following the addition of 1 mM 5-HT, with a 95% O2/5% CO2, gas phase, glucose uptake by H. diminuta was significantly (P less than 0.001) enhanced, remaining linear and parallel to that of control groups between 15 and 60 min incubation. Under air, the rates of glucose uptake were higher, but were only significantly increased by 5-HT during the first 30 min of incubation. In further experiments, in the absence of glucose in the incubation media worm glycogen reserves decreased by over 50% after 60 min. With the addition of 1 mM 5-HT, the reduction in glycogen content was significantly (P less than 0.025) greater, exceeding 65% after 60min. When glucose was added to the incubation media, worm glycogen reserves were not significantly depleted irrespective of the presence or absence of 5-HT. Incubations under a 95% O2/5% CO2 gas phase did not significantly influence glycogen content compared to corresponding groups incubated in air. The results suggest that 5-HT stimulates glycolysis in H. diminuta through increased glucose uptake by the worm or by a reduction in glycogen reserves in the absence of external glucose.
在含有10 mM葡萄糖的培养基中体外孵育后，微小膜壳绦虫对葡萄糖的摄取在长达60分钟内呈线性。在添加1 mM 5-羟色胺后，在95% O₂/5% CO₂气相条件下，微小膜壳绦虫对葡萄糖的摄取显著增强（P < 0.001），在孵育15至60分钟期间保持线性且与对照组平行。在空气条件下，葡萄糖摄取速率较高，但仅在孵育的前30分钟内5-羟色胺使其显著增加。在进一步实验中，孵育培养基中无葡萄糖时，60分钟后虫体糖原储备减少超过50%。添加1 mM 5-羟色胺后，糖原含量的减少显著更大（P < 0.025），60分钟后超过65%。当向孵育培养基中添加葡萄糖时，无论有无5-羟色胺，虫体糖原储备均未显著消耗。与在空气中孵育的相应组相比，在95% O₂/5% CO₂气相条件下孵育对糖原含量没有显著影响。结果表明，5-羟色胺通过增加虫体对葡萄糖的摄取或在无外源葡萄糖时减少糖原储备来刺激微小膜壳绦虫的糖酵解。
