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微小膜壳绦虫(绦虫纲)中的5-羟色胺、葡萄糖摄取、糖原利用及二氧化碳固定

5-hydroxytryptamine, glucose uptake, glycogen utilization and carbon dioxide fixation in Hymenolepis microstoma (Cestoda).

作者信息

Rahman M S, Mettrick D F, Podesta R B

出版信息

Comp Biochem Physiol B. 1982;73(4):901-6. doi: 10.1016/0305-0491(82)90334-0.

DOI:10.1016/0305-0491(82)90334-0
PMID:6817965
Abstract
  1. In vitro glucose uptake and glycogen utilization by Hymenolepis microstoma decreased under high oxygen concentrations. 2. 5-Hydroxytryptamine did not stimulate in vitro glucose uptake but did increase glycogen utilizations by H. microstoma. 3. The reduced glucose uptake under high oxygen concentrations (21 and 95%) resulted in a reduction in excretory products. 4. 14CO2-incorporation studies confirmed that, under both 95% O2:5% CO2 and air-minus-CO2 (identical to 21% O2). CO2-fixation by phosphoenolpyruvate carboxykinase (EC 4.1.1.32) was inhibited. 5. The specific activity of hexokinase (EC 2.7.1.1), phosphofructokinase (EC 2.7.1.11) and pyruvate kinase (EC 2.7.1.40) was not stimulated by 5-HT. 6. The concentration of ATP required for optimal stimulation of phosphofructokinase activity was 0.67 mM. Activity was further significantly increased by the addition of cAMP and even greater by AMP.
摘要
  1. 在高氧浓度下,微小膜壳绦虫的体外葡萄糖摄取和糖原利用减少。2. 5-羟色胺不刺激体外葡萄糖摄取,但确实增加了微小膜壳绦虫的糖原利用。3. 在高氧浓度(21%和95%)下葡萄糖摄取减少导致排泄产物减少。4. 14CO2掺入研究证实,在95% O2:5% CO2和无CO2空气(等同于21% O2)条件下,磷酸烯醇式丙酮酸羧激酶(EC 4.1.1.32)的CO2固定受到抑制。5. 己糖激酶(EC 2.7.1.1)、磷酸果糖激酶(EC 2.7.1.11)和丙酮酸激酶(EC 2.7.1.40)的比活性不受5-羟色胺刺激。6. 磷酸果糖激酶活性最佳刺激所需的ATP浓度为0.67 mM。添加环磷酸腺苷后活性进一步显著增加,添加腺苷一磷酸后增加幅度更大。

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