使用基于光泽精的化学发光法研究肺泡巨噬细胞功能。
Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.
作者信息
Williams A J, Cole P J
出版信息
Thorax. 1981 Nov;36(11):866-9. doi: 10.1136/thx.36.11.866.
Abstract
The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with those obtained using another method of detecting O2- release (r = 0.61; p less than 0.05) and are also related by regression analysis to polymorphonuclear leucocyte contamination of the alveolar macrophage suspension. This shows that alveolar macrophages produce lucigenin-dependent chemiluminescence of the same order of magnitude as do polymorphonuclear leucocytes.
摘要
本文描述了一种利用光泽精依赖性吞噬化学发光来评估肺泡巨噬细胞对调理素化颗粒或可溶性因子刺激的代谢活性的方法。超氧化物歧化酶抑制作用（95%）表明化学发光产生过程中需要超氧阴离子（O2-）。所获得的结果与使用另一种检测O2-释放的方法所获得的结果相关（r = 0.61；p小于0.05），并且通过回归分析也与肺泡巨噬细胞悬液中的多形核白细胞污染相关。这表明肺泡巨噬细胞产生的光泽精依赖性化学发光与多形核白细胞产生的化学发光量级相同。
相似文献
1
Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.使用基于光泽精的化学发光法研究肺泡巨噬细胞功能。
Thorax. 1981 Nov;36(11):866-9. doi: 10.1136/thx.36.11.866.
2
Human bronchoalveolar lavage cells and luminol-dependent chemiluminescence.人支气管肺泡灌洗细胞与鲁米诺依赖性化学发光
J Clin Pathol. 1981 Feb;34(2):167-71. doi: 10.1136/jcp.34.2.167.
3
In vitro stimulation of alveolar macrophage metabolic activity by polystyrene in the absence of phagocytosis.在无吞噬作用的情况下，聚苯乙烯对肺泡巨噬细胞代谢活性的体外刺激。
Br J Exp Pathol. 1981 Feb;62(1):1-7.
4
Lucigenin chemiluminescence and its relationship to mitochondrial respiration in phagocytic cells.
Biochem Biophys Res Commun. 1989 Mar 15;159(2):584-91. doi: 10.1016/0006-291x(89)90034-x.
5
Superoxide anion generation by alveolar inflammatory cells in simple pneumoconiosis and in progressive massive fibrosis of nonsmoking coal workers.非吸烟煤矿工人单纯尘肺和进行性大块纤维化中肺泡炎症细胞产生超氧阴离子的情况。
Am Rev Respir Dis. 1990 Jan;141(1):129-33. doi: 10.1164/ajrccm/141.1.129.
6
Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems.光泽精（双-N-甲基吖啶）作为化学发光探针用于检测酶促和细胞系统中超氧阴离子自由基生成的验证。
J Biol Chem. 1998 Jan 23;273(4):2015-23. doi: 10.1074/jbc.273.4.2015.
7
Release of superoxide anion by alveolar macrophages in pulmonary sarcoidosis.结节病患者肺泡巨噬细胞释放超氧阴离子的研究
Ann N Y Acad Sci. 1986;465:193-200. doi: 10.1111/j.1749-6632.1986.tb18495.x.
8
Halothane inhibits the microbicidal oxidative activity of pulmonary alveolar macrophages.
Anesthesiology. 1983 May;58(5):456-9. doi: 10.1097/00000542-198305000-00011.
9
Lucigenin as a substrate of microsomal NAD(P)H-oxidoreductases.光泽精作为微粒体NAD(P)H氧化还原酶的底物。
Biochemistry (Mosc). 1999 Jan;64(1):25-32.
10
Effect of ketotifen on human alveolar macrophages.
J Allergy Clin Immunol. 1988 Feb;81(2):469-74. doi: 10.1016/0091-6749(88)90919-0.
引用本文的文献
1
Enhancement of bronchoalveolar cell recovery and stimulation of alveolar macrophage chemiluminescence and resistance to influenza virus after treatment with RU 41821 aerosol.用RU 41821气雾剂治疗后支气管肺泡细胞恢复增强、肺泡巨噬细胞化学发光刺激及对流感病毒的抵抗力增强。
Antimicrob Agents Chemother. 1987 Jun;31(6):920-4. doi: 10.1128/AAC.31.6.920.
2
Increased release of free oxygen radicals by phagocytosing and nonphagocytosing cells from patients with active pulmonary sarcoidosis as revealed by luminol-dependent chemiluminescence.通过鲁米诺依赖的化学发光法揭示，来自活动性肺结节病患者的吞噬细胞和非吞噬细胞释放的游离氧自由基增加。
Klin Wochenschr. 1988 Apr 1;66(7):292-7. doi: 10.1007/BF01727514.
3
Number and activity of inflammatory cells in bronchoalveolar lavage fluid in asthma and their relation to airway responsiveness.哮喘患者支气管肺泡灌洗液中炎症细胞的数量和活性及其与气道反应性的关系。
Thorax. 1988 Sep;43(9):684-92. doi: 10.1136/thx.43.9.684.
4
Immunoglobulin E-dependent stimulation of human alveolar macrophages: significance in type 1 hypersensitivity.免疫球蛋白E依赖的人肺泡巨噬细胞刺激：在1型超敏反应中的意义。
Clin Exp Immunol. 1986 Aug;65(2):416-26.
5
Activated alveolar macrophages in subclinical pulmonary inflammation in collagen vascular diseases.胶原血管病亚临床肺部炎症中活化的肺泡巨噬细胞。
Thorax. 1988 Jan;43(1):24-30. doi: 10.1136/thx.43.1.24.
6
Nedocromil sodium inhibition of IgE-mediated activation of human mononuclear phagocytes and platelets from asthmatics.
Drugs. 1989;37 Suppl 1:32-6; discussion 69-77. doi: 10.2165/00003495-198900371-00008.
7
Effect of immunoglobulins on mineral dust-induced production of reactive oxygen metabolites by human macrophages.
Inflammation. 1990 Dec;14(6):621-9. doi: 10.1007/BF00916366.
本文引用的文献
1
Chemiluminescence induced by operation of iron-flavoproteins.铁黄素蛋白作用引发的化学发光
Biochemistry. 1962 Sep;1:779-83. doi: 10.1021/bi00911a008.
2
3
Oxygen toxicity.氧中毒
Am J Med. 1980 Jul;69(1):117-26. doi: 10.1016/0002-9343(80)90509-4.
4
Human bronchoalveolar lavage cells and luminol-dependent chemiluminescence.人支气管肺泡灌洗细胞与鲁米诺依赖性化学发光
J Clin Pathol. 1981 Feb;34(2):167-71. doi: 10.1136/jcp.34.2.167.
5
Cytochemical identification of monocytes and granulocytes.单核细胞和粒细胞的细胞化学鉴定。
Am J Clin Pathol. 1971 Mar;55(3):283-90. doi: 10.1093/ajcp/55.3.283.
6
Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.生物防御机制。白细胞产生超氧化物，一种潜在的杀菌剂。
J Clin Invest. 1973 Mar;52(3):741-4. doi: 10.1172/JCI107236.
7
Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages.鲁米诺依赖的化学发光法检测兔肺泡巨噬细胞和腹腔巨噬细胞的吞噬活性
Biochem Biophys Res Commun. 1976 Mar 8;69(1):245-52. doi: 10.1016/s0006-291x(76)80299-9.
8
Peroxidase activity of alveolar macrophages.肺泡巨噬细胞的过氧化物酶活性
Lab Invest. 1976 Jan;34(1):31-42.
9
10
The pulmonary-alveolar macrophage (first of two parts).肺泡巨噬细胞（两部分中的第一部分）
N Engl J Med. 1979 Sep 13;301(11):580-7. doi: 10.1056/NEJM197909133011104.
Zotero 插件