Hovmark A
Acta Derm Venereol. 1977;57(6):497-502.
T lymphocytes were stained in order to disclose alpha-naphthyl acetate esterase activity (ANAE staining) by adopting the method described by Mueller et al. In ANAE-staining of frozen sections from human lymph nodes, more than 90% of the lymphocytes in paracortical areas (T cell areas) were ANAE-positive, but in cortical follicles (B cell areas) less than 5% of the cells were positive. Lymphocytic infiltrations in various dermatoses (lichen ruber planus, psoriasis, SLE, atropic dermatitis, erythema multiforme, poikiloderma atrophicans vasculare, and Sézary syndrome) were investigated. A high percentage of ANAE-positive lymphoid cells (greater than 80%) was found in most cases. One patient with chron. lymphatic leukaemia, however, had a smaller proportion of ANAE-positive lymphocytic cells in an erythema multiforme skin infiltrate. ANAE staining seems to be an easy method for the identification of T lymphocytes in skin sections. The results of this investigation support the hypothesis that T lymphocytes have an affinity to skin.
采用穆勒等人描述的方法对T淋巴细胞进行染色，以揭示α-萘乙酸酯酶活性（ANAE染色）。在人淋巴结冰冻切片的ANAE染色中，副皮质区（T细胞区）90%以上的淋巴细胞ANAE呈阳性，而皮质滤泡（B细胞区）中阳性细胞不到5%。对各种皮肤病（扁平苔藓、银屑病、系统性红斑狼疮、萎缩性皮炎、多形红斑、血管萎缩性皮肤异色症和塞扎里综合征）中的淋巴细胞浸润情况进行了研究。大多数病例中发现高比例的ANAE阳性淋巴细胞（大于80%）。然而，1例慢性淋巴细胞白血病患者在多形红斑皮肤浸润中，ANAE阳性淋巴细胞的比例较小。ANAE染色似乎是一种在皮肤切片中识别T淋巴细胞的简便方法。本研究结果支持T淋巴细胞对皮肤有亲和力这一假说。
