Wunderlich V, Uckert W
Arch Virol. 1981;70(1):43-53. doi: 10.1007/BF01320792.
The protein patterns of type D retroviruses isolated either from permanent human cells (HeLa virus, HeLa V, and HEp-2 virus, HEp-2V) or from spectacled langur (langur virus, LV), an Old World monkey, were investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Labeling with 14C-amino acids and 3H-glucosamine revealed for each of these isolates five polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000 and about 68,000 and identified the 68,000 D-protein as a glycoprotein. An additional glycoprotein with a molecular weight of 20,000 (gp20) was resolved in LV similarly as in previous studies with Mason-Pfizer monkey virus. However, in accordance with our recent analysis of a third type D isolate from human cells (PMFV), gp20 was not detectable in both HeLaV and HEp-2V regardless of the cell line in which they were grown. Thus, it appears that the type D virus isolates from human cells are slightly distinct from the presently known type D retroviruses of Old World monkeys. The relevance of this finding to the origin of the human isolates, however, remains to be shown.
利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法,对从人永生细胞(海拉病毒、海拉V和人喉表皮样癌细胞病毒、HEp-2V)或来自旧大陆猴眼镜猴(叶猴病毒、LV)中分离出的D型逆转录病毒的蛋白质模式进行了研究。用¹⁴C-氨基酸和³H-葡糖胺标记显示,这些分离株中的每一种都有五条分子量分别为10000、12000、15000、25000和约68000的多肽,并确定68000的D蛋白为糖蛋白。与先前对梅森- Pfizer猴病毒的研究类似,在LV中解析出了一条分子量为20000的额外糖蛋白(gp20)。然而,根据我们最近对来自人细胞的第三种D型分离株(PMFV)的分析,无论海拉V和HEp-2V在何种细胞系中生长,都检测不到gp20。因此,似乎从人细胞中分离出的D型病毒分离株与目前已知的旧大陆猴D型逆转录病毒略有不同。然而,这一发现与人类分离株起源的相关性仍有待证明。