Influence of puromycin and chloramphenicol on aminoketone synthesis ( delta-aminolevulinic acid synthesis) induction by allylisopropylacetamide in rat liver.

In vitro induction of aminoketone synthesis (AKS) by allylisopropylacetamide (AIA) in liver slices from 30 day-old male rats was reversibly inhibited by puromycin (50 micrograms/ml incubation mixture). Normal AKS was initially stimulated and thereafter blocked. Chloramphenicol (350 micrograms/ml) inhibited AIA mediated induction of AKS only partially and only when added 1 h before AIA to the incubation mixture. In vivo puromycin (3 i.p. injections of 15 mg/kg in 1 h intervals) did not influence AKS or AIA mediated AKS induction in newborn rats, whereas chloramphenicol (500 mg/kg s.c. 30 min prior to AIA) inhibited AIA mediated AKS induction in newborn rats. In 40 day-old rats pretreatment with chloramphenicol completely inhibited AKS induction by 200 mg/kg AIA.