Reyes A A, Johnson M J, Schöld M, Ito H, Ike Y, Morin C, Itakura K, Wallace R B
Immunogenetics. 1981;14(5):383-92. doi: 10.1007/BF00373318.
Based on the published amino-acid sequence of H-2Kb, we synthesized a mixture of eight 16-base long oligodeoxyribonucleotides representing all possible coding sequences for residues 51-56 (Trp-Met-Glu-Gln-Glu-Gly). The hexadecanucleotide mixture was used as a probe to screen recombinant DNA clones constructed from cytoplasmic PolyA+ RNA isolated from the murine thymoma cell line EL4(b haplotype). Of the 30 000 independent clones screened, one clone was found to hybridize with the probe. DNA sequence analysis showed that the cDNA clone was derived from a portion of an H-2Kb -related mRNA. The clone encodes a protein sequence identical with a region of H-2Kb in 42 consecutive residues (50 through 91). The sequence than diverges from the H-2Kb sequence and, after a single Glu codon, a termination codon is encountered. It is possible that this mRNA codes for a small 92 amino-acid protein with a sequence identical (except for a carboxy-terminal Glu residue) with the amino terminus of H-2Kb. It is further speculated that this mRNA is coded for by the H-2Kb gene and differs from the H-2Kb mRNA in the pattern of posttranscriptional splicing.
根据已发表的H-2Kb氨基酸序列，我们合成了一个由八个16碱基长的寡脱氧核糖核苷酸组成的混合物，它们代表了残基51 - 56（色氨酸-甲硫氨酸-谷氨酸-谷氨酰胺-谷氨酸-甘氨酸）的所有可能编码序列。该十六核苷酸混合物用作探针，以筛选从鼠胸腺瘤细胞系EL4（b单倍型）分离的细胞质聚腺苷酸加尾RNA构建的重组DNA克隆。在筛选的30000个独立克隆中，发现一个克隆与探针杂交。DNA序列分析表明，该cDNA克隆源自与H-2Kb相关的mRNA的一部分。该克隆编码的蛋白质序列在42个连续残基（50至91）中与H-2Kb的一个区域相同。该序列随后与H-2Kb序列 diverges ，并且在单个谷氨酸密码子之后，遇到一个终止密码子。有可能该mRNA编码一种92个氨基酸的小蛋白质，其序列（除了羧基末端的谷氨酸残基）与H-2Kb的氨基末端相同。进一步推测该mRNA由H-2Kb基因编码，并且在转录后剪接模式上与H-2Kb mRNA不同。 （注：diverges这个词在原文中可能有误，推测可能是“diverges”，意为“分歧、偏离”，按此理解翻译了该句）
