Reyes A A, Schöld M, Wallace R B
Immunogenetics. 1982;16(1):1-9. doi: 10.1007/BF00364437.
A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3'-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3'-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3'-coding ends.
用一个含有H - 2Kb编码区一小部分的探针筛选了来自EL4细胞系（b单倍型）的小鼠cDNA文库。分离得到的一个克隆，pH203，编码一种蛋白质，其推导的氨基酸序列在141个可比较的位置中有141个与已知的H - 2Db序列相同。因此，该克隆被认为编码H - 2Db移植抗原。pH203的cDNA插入片段包含H - 2Db第82位残基到羧基末端的编码区，并包括476个核苷酸的3' - 非翻译序列。H - 2Db cDNA克隆与先前分离的H - 2Kb cDNA克隆之间的比较显示，在氨基酸和核苷酸水平上的同源性分别为83%和91%。对3' - 编码区和非翻译区的DNA序列分析表明，H - 2Kb和H - 2Db的mRNA在其3' - 编码末端的剪接方式不同。
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