Torinuku W, Miura T
Tohoku J Exp Med. 1981 Dec;135(4):387-93. doi: 10.1620/tjem.135.387.
The intensity of ultraweak chemiluminescence (CL) was estimated in rat epidermal homogenate with a technique utilizing UV irradiation (in vitro and in vivo). CL markedly increased in the homogenate after irradiation. The CL intensity was enhanced to 300% by the substitution of deuterium oxide for H2O, and it was reduced to 50% when diazabicyclo-octane, a scavenger for singlet oxygen, was added. It was concluded that the weak CL would be attributable to singlet oxygen, and the determination of the lipoperoxide in skin tissue by the method of the CL counting system was discussed.
利用紫外线照射技术(体外和体内)对大鼠表皮匀浆中的超微弱化学发光(CL)强度进行了评估。照射后匀浆中的CL显著增加。用重水替代H2O后,CL强度增强至300%,而当加入单线态氧清除剂二氮杂双环辛烷时,CL强度降低至50%。得出结论,微弱的CL可能归因于单线态氧,并讨论了通过CL计数系统方法测定皮肤组织中的脂质过氧化物。