Miyazawa T, Kaneda T
J Nutr Sci Vitaminol (Tokyo). 1981;27(5):415-23. doi: 10.3177/jnsv.27.415.
Extra-weak chemiluminescence was detected in the organ homogenate and blood of tocopherol-deficient rats by use of a newly devised single photon counting apparatus. The spectrum distribution showed that the chemiluminescence had emission peaks at wavelengths between 500 and 650 nm corresponding to the simultaneous transition of singlet molecular oxygen (1O2), 2[1 delta g] leads to 2[3 epsilon g-]. This chemiluminescence was quenched by the presence of free radical scavengers, butyl hydroxytoluene and d-alpha-tocopherol. It was stimulated by D2O and 1O2-emission enhancer, 1,4-diazabicyclo[2,2,2]octane. The chemiluminescent intensities in tissue of rats fed a tocopherol-free diet for seven months were higher than those of rats fed a normal diet which contained 15 mg of tocopherol per 100 g of diet. The considerable increase of light emission was observed especially in liver, kidney, heart, lung and brain homogenates. The intensity of tocopherol-deficient liver chemiluminescence corresponded to 22 x 10(3) photons per sec . cm2. The results indicated that the chemiluminescence was directly related to the generation of 1O2 involving free radical reactions in the tocopherol-deficient rat tissues.
使用新设计的单光子计数装置，在生育酚缺乏的大鼠的器官匀浆和血液中检测到了极微弱的化学发光。光谱分布表明，该化学发光在500至650纳米波长处有发射峰，对应于单线态分子氧（1O2）的同时跃迁，即2[1δg] 转变为2[3εg-]。这种化学发光被自由基清除剂丁基羟基甲苯和d-α-生育酚淬灭。它受到重水和1O2发射增强剂1,4-二氮杂双环[2,2,2]辛烷的刺激。喂食不含生育酚的饮食七个月的大鼠组织中的化学发光强度高于喂食每100克饮食中含有15毫克生育酚的正常饮食的大鼠。尤其在肝脏、肾脏、心脏、肺和脑匀浆中观察到发光显著增加。生育酚缺乏的肝脏化学发光强度相当于每秒每平方厘米22×10(3) 个光子。结果表明，这种化学发光与生育酚缺乏的大鼠组织中涉及自由基反应的1O2的产生直接相关。
