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[淋巴细胞低温保存中的三磷酸腺苷、二磷酸腺苷和一磷酸腺苷含量]

[ATP, ADP and AMP content in the low-temperature preservation of lymphoid cells].

作者信息

Gordienko A D, Fedets O I

出版信息

Vopr Med Khim. 1981 Nov-Dec;27(6):746-9.

PMID:7336648
Abstract

As compared with ATP and ADP concentration in native lymphocytes, content of ATP was decreased down to 26.3% and 35.1% and of ADP--37.0% and 44.4% in the cell preparations after conservation in mixtures containing 10% PEO-400 and dimethyl-sulfoxide (DMSO). AMP was not found in the lymphocytes when both PEO-400 and DMSO were used. Mechanisms responsible for instability of lymphocyte adenine nucleotides under conditions of low temperature conservation are discussed.

摘要

与天然淋巴细胞中的ATP和ADP浓度相比,在含有10%聚乙二醇-400(PEO-400)和二甲基亚砜(DMSO)的混合物中保存后的细胞制剂中,ATP含量分别降至26.3%和35.1%,ADP含量分别降至37.0%和44.4%。当同时使用PEO-400和DMSO时,淋巴细胞中未检测到AMP。文中讨论了低温保存条件下淋巴细胞腺嘌呤核苷酸不稳定的相关机制。

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