不同时长食物剥夺对下丘脑外侧区5-羟色胺代谢的影响。

Effects of various periods of food deprivation on serotonin turnover in the lateral hypothalamus.

作者信息

Kantak K M, Wayner M J, Stein J M

出版信息

Pharmacol Biochem Behav. 1978 Oct;9(4):529-34. doi: 10.1016/0091-3057(78)90054-0.

Abstract

Preliminary results indicated enhanced serotonin turnover in the lateral hypothalamus of 24 hr food deprived rats as compared to non-deprived rats. In the present study, the periods of food deprivation were extended in ordered that the effects of 0. 24, 48 and 72 hr of food deprivation on serotonin turnover could be measured. One hr following an infusion of 3H-5-hydroxytryptamine the lateral hypothalamus was perfused with physiological bacteriostatic saline for 40 min. Samples of perfusate, which corresponds to 75--90 min post-infusion, were analyzed by thin layer chromatography for estimation of 3H-labelled precursor and metabolites. The results indicate that serotonin turnover is enhanced as a function of hours of food deprivation.

摘要

初步结果表明，与未禁食的大鼠相比，禁食24小时的大鼠下丘脑外侧的5-羟色胺更新增强。在本研究中，延长禁食时间，以便能够测定禁食0、24、48和72小时对5-羟色胺更新的影响。注入3H-5-羟色胺1小时后，用生理抑菌盐水灌注下丘脑外侧40分钟。对相当于注入后75-90分钟的灌注液样本进行薄层色谱分析，以估计3H标记的前体和代谢物。结果表明，5-羟色胺更新随禁食时间的延长而增强。