de Lisle G W, Duncan J R
Can J Comp Med. 1981 Jul;45(3):304-9.
A whole blood lymphocyte transformation test was used to examine cattle with varying degrees of infection with Mycobacterium paratuberculosis. Minimally infected animals characteristically responded to johnin purified protein derivative in the lymphocyte transformation test but did not routinely react on serological and/or skin testing. Heavily infected animals showed considerable variation in their lymphocyte transformation responses to antigen and some of them were consistently unresponsive. Antigen induced lymphocyte transformation reactions were recorded in 7.6 to 41.5% of uninfected animals whose infection status was determined by bacteriology and/or histopathology. The number of positive reactions recorded in uninfected animals depended on the population, the larger percentage being found in a herd with a proven history of paratuberculosis. The potential of lymphocyte transformation as a diagnostic test for bovine paratuberculosis is discussed.
采用全血淋巴细胞转化试验对不同程度感染副结核分枝杆菌的牛进行检测。轻度感染的动物在淋巴细胞转化试验中对副结核菌素纯蛋白衍生物有典型反应，但在血清学和/或皮肤试验中通常无反应。重度感染的动物对抗原的淋巴细胞转化反应差异很大，其中一些动物始终无反应。通过细菌学和/或组织病理学确定感染状况的未感染动物中，有7.6%至41.5%出现抗原诱导的淋巴细胞转化反应。未感染动物中阳性反应的数量取决于群体，在有副结核病确诊病史的牛群中发现的阳性反应百分比更高。本文讨论了淋巴细胞转化作为牛副结核病诊断试验的潜力。
