Cunliffe-Beamer T L, Freeman L C, Myers D D
Lab Anim Sci. 1981 Dec;31(6):672-5.
Effect of unautoclaved or autoclaved contact bedding materials (mixed hardwood, white spruce, white pine, and red cedar shavings) on pentobarbital sleeptime and liver:body weight ratios was assayed in young DBA/2J and C57BL/6J male mice. Analysis of variance indicated that autoclaving the bedding did not alter either parameter in either strain. Within strains, data were pooled and analyzed for bedding effects. Sleeptimes of DBA/2J male mice on mixed hardwood or white spruce bedding were not significantly different, but were significantly longer than sleeptimes of mice on white pine or red cedar bedding. Sleeptimes of DBA/2J mice on white pine were significantly longer than mice on red cedar. Sleeptimes on C57BL/6J male mice on each bedding were significantly different with the following rank order:mixed hardwood greater than white spruce greater than white pine greater than red cedar. In both strains, liver:body weight ratios of mice on red cedar bedding were significantly increased compared to mice on white pine, white spruce, or mixed hardwood beddings. The results of this study indicated that autoclaving these beddings did not alter pentobarbital sleeptimes or liver/:body weight ratios and that softwood beddings differ in their abilities to alter pentobarbital sleeptimes.
在年轻的DBA/2J和C57BL/6J雄性小鼠中，检测了未高压灭菌或高压灭菌的接触垫料（混合硬木、白云杉、白松和红雪松刨花）对戊巴比妥睡眠时间和肝体重比的影响。方差分析表明，对垫料进行高压灭菌不会改变任一品系的任何一个参数。在各品系内，汇总数据并分析垫料的影响。DBA/2J雄性小鼠在混合硬木或白云杉垫料上的睡眠时间没有显著差异，但显著长于在白松或红雪松垫料上的小鼠睡眠时间。DBA/2J小鼠在白松垫料上的睡眠时间显著长于在红雪松垫料上的。C57BL/6J雄性小鼠在每种垫料上的睡眠时间有显著差异，顺序如下：混合硬木大于白云杉大于白松大于红雪松。在两个品系中，与在白松、白云杉或混合硬木垫料上的小鼠相比，在红雪松垫料上的小鼠肝体重比显著增加。本研究结果表明，对这些垫料进行高压灭菌不会改变戊巴比妥睡眠时间或肝体重比，且软木垫料在改变戊巴比妥睡眠时间的能力上存在差异。
