Roth J A, Kaeberle M L
Vet Immunol Immunopathol. 1981 Apr;2(2):157-74. doi: 10.1016/0165-2427(81)90047-7.
Bovine polymorphonuclear leukocytes (PMNs) were isolated from the peripheral blood of cattle. Five in vitro procedures were utilized to evaluate PMN function: 1) Random migration under agarose, 2) Ingestion of 125I-iododeoxyuridine labeled Staphylococcus aureus, 3) Quantitative nitroblue tetrazolium reduction, 4) Chemiluminescence and 5) Iodination. Normal values for bovine PMNs are reported and interpretation of results is discussed. The PMN function tests were designed so that all 5 procedures may be performed in a short period of time on the same cell preparation. This allows for the detection and partial characterization of a potential PMN dysfunction.
牛多形核白细胞（PMNs）从牛的外周血中分离出来。采用了五种体外实验方法来评估PMN的功能：1）琼脂糖下的随机迁移；2）摄取125I-碘脱氧尿苷标记的金黄色葡萄球菌；3）定量硝基蓝四氮唑还原实验；4）化学发光实验；5）碘化实验。报告了牛PMNs的正常值并讨论了结果的解释。PMN功能测试的设计使得所有这五种实验方法都可以在短时间内对同一细胞制剂进行。这有助于检测潜在的PMN功能障碍并进行部分特征描述。
