A型多杀性巴氏杆菌组分对牛多形核白细胞功能的影响。
Effect of type A Pasteurella multocida fractions on bovine polymorphonuclear leukocyte functions.
作者信息
Ryu H, Kaeberle M L, Roth J A, Griffith R W
出版信息
Infect Immun. 1984 Jan;43(1):66-71. doi: 10.1128/iai.43.1.66-71.1984.
The effect of various Pasteurella multocida fractions on bovine polymorphonuclear leukocyte (PMN) functions was examined in vitro by using two encapsulated strains, P-2383 and P-1062 (both are Carter capsular type A and of bovine origin). The ability of PMNs to ingest Staphylococcus aureus and iodinate protein was significantly inhibited in the presence of live cells, heat-killed whole cells, or saline-extracted capsules but not in the presence of the decapsulated heat-killed cells. None of the fractions of the two strains inhibited nitroblue tetrazolium reduction by PMNs. The saline extract did not inhibit the binding of iodine to protein by a reaction involving xanthine, xanthine oxidase, and horseradish peroxidase. The PMN inhibitory factor was further characterized as a heat-stable capsular material of greater than 300,000 molecular weight.
通过使用两种荚膜菌株P-2383和P-1062（均为卡特荚膜A型且源自牛），在体外检测了多杀性巴氏杆菌不同组分对牛多形核白细胞（PMN）功能的影响。在活细胞、热灭活全细胞或盐提取荚膜存在的情况下，PMN摄取金黄色葡萄球菌和碘化蛋白质的能力受到显著抑制，但在脱荚膜热灭活细胞存在的情况下则未受抑制。两种菌株的任何组分均未抑制PMN对硝基蓝四唑的还原。盐提取物未通过涉及黄嘌呤、黄嘌呤氧化酶和辣根过氧化物酶的反应抑制碘与蛋白质的结合。PMN抑制因子进一步被鉴定为分子量大于300,000的热稳定荚膜物质。
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